Mouse Anti-Serpinh1 Recombinant Antibody (VS3-FY2870)
CAT#: VS3-FY2870
This anti-Serpinh1 antibody is a mouse recombinant monoclonal antibody. It has been specifically designed to target the human, mouse Serpinh1 protein, a calculated molecular weight of the protein is about 46.5 kDa. This monoclonal antibody proves valuable in disease research related to Osteogenesis Imperfecta, Type X and Preterm Premature Rupture of The Membranes. The antibody can be utilized for detection of Serpinh1 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29198/1866_A1_36_selected.jpg
Normal Tissue
Figure 2 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows moderate cytoplasmic positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29198/63002_B_6_8_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (CACO-2, fHDF/TERT166, HHSteC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149257-SERPINH1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human and Mouse Serpinh1
- Species Reactivity
- Human, Mouse
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 46.5 kDa.
- Related Disease
- Osteogenesis Imperfecta, Type X; Preterm Premature Rupture of The Membranes
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Target
- Alternative Names
- Serpin Family H Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Cell Proliferation-Inducing Gene 14 Protein; Rheumatoid Arthritis-Related Antigen RA-A47; Arsenic-Transactivated Protein 3; Collagen Binding Protein 1; 47 KDa Heat Shock Protein; SERPINH2; AsTP3; HSP47; CBP1; CBP2; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2)
- Gene ID
- 871
- UniProt ID
- P50454
- Cellular Localization
- Endoplasmic reticulum lumen
- Post Translation Modifications
- Glycosylation at Asn120 and Asn125 (NX_P50454 [NX_P50454-1])
Ubiquitination at Lys39, Lys94, Lys319, and Lys380 (NX_P50454 [NX_P50454-1])
Modification sites at PhosphoSitePlus (P50454)
Glycosylation from GlyConnect SERPH_HUMAN (658)
Glycosylation from GlyGen (P50454) 6 sites, 28 N-linked glycans (2 sites), 1 O-linked glycan (4 sites)
- Protein Refseq
- XP_024304524.1
- Function
- Binds specifically to collagen.
Could be involved as a chaperone in the biosynthetic pathway of collagen.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SERPINH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2872z | Mouse Anti-SERPINH1 Recombinant Antibody (clone 1B4) | WB, ICC, IF | Mouse IgG1, κ |
| MOB-0879CT | Recombinant Mouse anti-Human SERPINH1 Monoclonal antibody (EML235) | ICC, IF, IHC-P, WB | |
| MRO-1943-CN | Rabbit Anti-SERPINH1 Polyclonal Antibody (MRO-1943-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0559U | Rabbit Anti-SERPINH1 Recombinant Antibody (clone 10B2) | ELISA, IHC | Rabbit IgG |
| VS3-XY3501 | Rabbit Anti-SERPINH1 Recombinant Antibody (clone R09-6D6) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3228 | Hi-Affi™ Recombinant Rabbit Anti-SERPINH1 Monoclonal Antibody (DS3228AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6499 | Anti-SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6500 | Anti-Rat SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6501 | Anti-Mouse SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6502 | Anti-Hamster SERPINH1 Immunohistochemistry Kit | IHC |
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