Rabbit Anti-SF3A3 Recombinant Antibody (clone R06-3I7)
CAT#: VS3-FY1944
Clone R06-3I7 is a rabbit recombinant monoclonal antibody that has a high affinity for human SF3A3. It cross-reacts with mouse, rat, hamster. Applications Tested: The SF3A3 antibody has been tested by western blot analysis of SF3A3 in 3T3, Raw264.7, C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-SF3A3 Antibody (VS3-FY1944) in WB.
The SF3A3 expression in 3T3, Raw264.7, C6 lysates were detected using the anti-SF3A3 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/378_E3_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_9_7_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_B_8_5.jpg
Normal Tissue
Figure 5 Oral mucosa
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_7_1.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32054/70658_A_5_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183431-SF3A3
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Specifications
- Immunogen
- Synthetic peptide of human SF3A3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster SF3A3
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R06-3I7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Splicing Factor 3a Subunit 3; Splicing Factor 3a, Subunit 3, 60kDa; Splicing Factor 3a, Subunit 3, 60kD; SAP 61; SF3a60; SAP61
- Gene ID
- 10946
- UniProt ID
- Q12874
- Sequence Similarities
- Belongs to the SF3A3 family.
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys201 and Lys390 (NX_Q12874 [NX_Q12874-1])
Modification sites at PhosphoSitePlus (Q12874)
Glycosylation from GlyGen (Q12874) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001307759.1; NP_006793.1
- Function
- The 17S U2 SnRNP complex 1 directly participates in early spliceosome assembly and 2 mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SF3A3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1877CT | Recombinant Mouse anti-Human SF3A3 Monoclonal antibody (EML1173) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3239 | Hi-Affi™ Recombinant Rabbit Anti-SF3A3 Monoclonal Antibody (DS3239AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2045 | Anti-SF3A3 Immunohistochemistry Kit | IHC |
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