Mouse Anti-SGK3 Recombinant Antibody (clone 2506W45)
CAT#: VS-0625-FY77
This Mouse IgG2b kappa monoclonal antibody, clone 2506W45, specifically targets SGK3 in Human, Mouse, and Rat. Its immunogen was raised against amino acids 67-145 of human SGK3. With an observed antigen size of about 37 kDa (calculated 51.58 kDa), this antibody is applicable in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and ELISA, offering broad utility for SGK3 research.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6699/1758_C3_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27146/56377_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104205-SGK3
❮
❯
Specifications
- Immunogen
- Antibody raised against human SGK3, targeting amino acids 67-145 near the N-terminus.
- Host Species
- Mouse
- Type
- Mouse IgG2b, kappa
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2506W45
- Applications
- Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry-Paraffin, ELISA
- Related Disease
- Immunodeficiency 55 and Hypophosphatemic Rickets
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Serum/Glucocorticoid Regulated Kinase Family Member 3; Cytokine-Independent Survival Kinase; EC 2.7.11.1; SGKL; CISK; Serum/Glucocorticoid Regulated Kinase Family, Member 3; Serum/Glucocorticoid Regulated Kinase-Like
- Gene ID
- 23678
- UniProt ID
- Q96BR1
- Long Name
- Serum/Glucocorticoid Regulated Kinase Family Member 3
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
- Cellular Localization
- Cytoplasmic vesicle {ECO:0000250}
Early endosome
Recycling endosome
- Post Translation Modifications
- Activated by phosphorylation on Ser-486 by an unknown kinase, transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- NP_001028750.1; NP_037389.4; NP_733827.2
- Function
- Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration.
Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0625-FY77. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SGK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0760MZ | Chicken Anti-SGK3 Polyclonal IgY | ELISA, IC, IF, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6543 | Anti-SGK3 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
