Monkey Anti-SIV gp41 Recombinant Antibody (B408) (CAT#: HPAB-0717-YJ)

Figure 1 Ability of Fabs to bind virus-infected cells.

Percentages of cell-surface Fab⁺ cells without permeabilization (upper) and Fab⁺ cells after staining both surface and intracellular staining by permeabilization (lower) are shown.

Kuwata, T., Katsumata, Y., Takaki, K., Miura, T., & Igarashi, T. (2011). Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display. AIDS research and human retroviruses, 27(5), 487-500.

Figure 2 Ability of Fabs to bind virus-infected cells.

Flow cytometry profifiles of PM1 cells infected with SIVsmE543-3, SIVmac239, and HIV-2GH123 are shown as representative samples. The results of surface staining were shown in Fab clones against gp120 and gp41, and those of surface and intracellular staining were shown in Anti-p27 Fab, as indicated on the right. The tinted histogram represents cells stained by the Fab indicated on the left. The dotted line shows unstained control.

Kuwata, T., Katsumata, Y., Takaki, K., Miura, T., & Igarashi, T. (2011). Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display. AIDS research and human retroviruses, 27(5), 487-500.

Figure 3 Anti-gp120 Fabs, B404, B406, B433, and B448, neutralized various SIV strains.

Anti-gp120 Fabs, B404, B406, B433, and B448, neutralized various SIV strains. Neutralizing activity against SIVsmH635 (A), SIVsmE543-3 (B), SIVmac239 (C), SIVmac316 (D), and HIV-2GH123 (E) was measured using Fabs (left panels) and plasma samples (right panels). Titration curves of percent inhibition of virus infectivity by anti-gp120 Fab clones, B404 (circles), B406 (squares), B433 (diamonds), and B448 (inverted triangles), are shown with anti-gp41 Fab B408 (triangles) as a negative control. A plasma sample from SIVsmH635-infected macaque H723 (black circles), from which the phage library was constructed, is compared with that from normal macaque MM464 (crosses). The 50% and 90% inhibitory doses are shown by the dotted line.

Kuwata, T., Katsumata, Y., Takaki, K., Miura, T., & Igarashi, T. (2011). Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display. AIDS research and human retroviruses, 27(5), 487-500.

Figure 4 Identification of epitope specificity of anti-gp120 Fab clones by competition ELISA.

(A) IgG-B404 inhibited the binding of anti-gp120 Fabs, B404, B406, B433, and B448, but did not inhibit anti-gp41 Fab B406. (B) IgG-B404 competed with the binding of murine MAbs, KK42, KK45, and KK46, which recognize a linear epitope in the V3 loop of gp120. (C) IgG-B404 did not compete with murine MAbs, KK8 (V1/V2), KK17 (AA8-303), KK65 (V1), KK68 (C1), and VM.18S (unknown).

Kuwata, T., Katsumata, Y., Takaki, K., Miura, T., & Igarashi, T. (2011). Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display. AIDS research and human retroviruses, 27(5), 487-500.