Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F)
CAT#: MOB-0057F
This product is a mouse antibody that recognizes SMARCB1.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F) in IHC
Immunohistochemical analysis of paraffin-embedded astrocytoma. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH 6.0 citrate buffer for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F) in IHC
High magnification immunohistochemical analysis of paraffin-embedded brain. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Use pH 6.0 citrate buffer for antigen retrieval. 3. Dilute the secondary antibody at 1:200 (room temperature, 30min).
WB
Figure 3 Mouse Anti-SMARCB1 Recombinant Antibody (MOB-0057F) in WB
Western blot analysis of INI-1 Antibody at a dilution of 1:1000.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/144_B9_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_8_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_7_5.jpg
Normal Tissue
Figure 8 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18248/40923_A_4_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099956-SMARCB1
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Specifications
- Immunogen
- Synthesized peptide derived from human INI-1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMARCB1
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
- Alternative Names
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1; Protein Phosphatase 1, Regulatory Subunit 144; Sucrose Nonfermenting, Yeast, Homolog-Like 1; Malignant Rhabdoid Tumor Suppressor; Integrase Interactor 1 Protein; BRG1-Associated Factor 47; SNF5 Homolog; SNF5L1; BAF47; HSNF5; INI1; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1; SWI/SNF-Related Matrix-Associated Protein
- Gene ID
- 6598
- UniProt ID
- Q12824
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMARCB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4029z | Mouse Anti-SMARCB1 Recombinant Antibody (clone 21H6) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1415-CN | Recombinant Rabbit Anti-SMARCB1 Monoclonal Antibody (JB49-18) | WB, IHC, FC | Rabbit IgG |
| VS3-WK908 | Mouse Anti-SMARCB1 Recombinant Antibody (clone ABT-INI1) | IHC-P, WB | Mouse IgG |
| VS3-FY1362 | Recombinant Mouse Anti-SMARCB1 Antibody (clone 5F1-6F6-3A1) | IHC-P | Mouse IgG |
| VS3-XY3509 | Rabbit Anti-SMARCB1 Recombinant Antibody (clone R06-2C2) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3330 | Hi-Affi™ Recombinant Rabbit Anti-SMARCB1 Monoclonal Antibody (DS3330AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6735 | Anti-SMARCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6736 | Anti-Mouse SMARCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6737 | Anti-Rat SMARCB1 Immunohistochemistry Kit | IHC |
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