Rabbit Anti-SMC1A Recombinant Antibody (clone 8C11)
CAT#: ZG-0571U
Rabbit Anti-SMC1A Antibody (clone 8C11) displayed high specificity against the SMC1A protein of Human.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-SMC1A Antibody (ZG-0571U) in IF
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate
All lanes: SMC1A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 144 kDa
Observed band size: 144 kDa
IHC
Figure 2 Rabbit Anti-SMC1A Antibody (ZG-0571U) in IHC
IHC image of ZG-0571U diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-SMC1A Antibody (ZG-0571U) in IF
IHC image of ZG-0571U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_8_5.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_4_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25404/54572_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000072501-SMC1A
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Specifications
- Immunogen
- A synthesized peptide derived from human SMC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SMC1A
- Species Reactivity
- Human
- Clone
- 8C11
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Developmental And Epileptic Encephalopathy 85 With Or Without Midline Brain Defects and Cornelia De Lange Syndrome 2.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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