Rabbit Anti-SNRPC Recombinant Antibody (clone R09-6E9) (CAT#: VS3-FY1926)
This R09-6E9 recombinant monoclonal antibody exhibits high affinity to human SNRPC. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the U1C antibody was determined using western blot analysis of U1C in rat lung lysates.
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WB
Figure 1 Anti-SNRPC Antibody (VS3-FY1926) in WB.
The U1C expression in rat lung lysates were detected using the anti-U1C by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human U1-C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat SNRPC
- Species Reactivity
- Human, Rat
- Clone
- R09-6E9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 21 kDa.
- Related Disease
- Connective tissue diseases
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Small Nuclear Ribonucleoprotein Polypeptide C; U1 SnRNP C; U1C; U1 Small Nuclear Ribonucleoprotein C; U1 Small Nuclear RNP Specific C; U1 SnRNP Protein C; Yhc1; U1-C
- Gene ID
- 6631
- UniProt ID
- P09234
- Sequence Similarities
- Belongs to the U1 small nuclear ribonucleoprotein C family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys3, Lys40, and Lys52 (NX_P09234 [NX_P09234-1])
Modification sites at PhosphoSitePlus (P09234)
Glycosylation from GlyGen (P09234) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_003084.1
- Function
- Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2439z | Mouse Anti-SNRPC Recombinant Antibody (clone 27B9) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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