Anti-SP140 Antibody Prodrug, Protease Activated (02J54)
CAT#: VS-1025-YC136
The Anti-SP140 Antibody Prodrug, Protease Activated (02J54) is an antibody prodrug comprised of a recombinant human antibody targeting human SP140, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to SP140 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to SP140 effectively. SP140 is a nuclear protein whose primary relevance to oncology comes from its role as an autoantigen and its mutations in chronic lymphocytic leukemia (CLL). Its involvement in chromatin remodeling and gene regulation suggests it acts as a tumor suppressor, with its loss contributing to malignancy.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67493/1397_C4_4_selected.jpg
Normal Tissue
Figure 2 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67493/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67493/154084_A_7_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079263-SP140
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Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human SP140
- Target
- SP140
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- 02J54
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants.
- Alternative Names
- LYSP100; LYSP100-A; LYSP100-B
- Gene ID
- 11262
- UniProt ID
- Q13342
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone 02J54"
- CAT
- Product Name
See other products for "SP140"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABS-0254 | Human Anti-SP140 Recombinant Antibody (clone 02J54) | IP, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-441C | Human Anti-SP140 Recombinant Antibody (ZG-441C) | ELISA | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6839 | Anti-SP140 Immunohistochemistry Kit | IHC |
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