AbPlus™ Anti-Sparc Magnetic Beads (VS-0724-YC635) (CAT#: VS-0724-YC635)
The AbPlus Anti-Sparc Magnetic Beads (VS-0724-YC635) is an innovative affinity resin which is bound with anti-Sparc specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of Sparc protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
Figure 6 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-Sparc specific antibody
- Target
- Sparc
- Immunogen
- Recombinant mouse Osteonectin/SPARC, aa 1-302.
- Target Species
- Mouse
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Enables calcium ion binding activity and extracellular matrix binding activity. Acts upstream of or within several processes, including bone development; cellular response to growth factor stimulus; and pigmentation. Located in extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in cervix carcinoma; endometrial carcinoma; and osteogenesis imperfecta type 17.
- Alternative Names
- ON; BM-40
- Gene ID
- 20692
- UniProt ID
- P07214
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SPARC"
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IgG Antibody Products
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3572z | Mouse Anti-SPARC Recombinant Antibody (clone 41H5) | WB, IP, IF, IHC | Mouse IgG1 |
| MOB-0379MZ | Recombinant Mouse Anti-Human Osteonectin Antibody (clone BPO-5032) | RIA, WB, ELISA | Mouse antibody |
| HPAB-0267-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 2) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0270-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 1) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0282-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 5) | WB, ELISA | Humanized IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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