AbPlus™ Anti-Sparc Magnetic Beads (VS-0724-YC635) (CAT#: VS-0724-YC635)
The AbPlus Anti-Sparc Magnetic Beads (VS-0724-YC635) is an innovative affinity resin which is bound with anti-Sparc specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of Sparc protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg

Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg

Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg

Figure 6 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg

Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-Sparc specific antibody
- Target
- Sparc
- Immunogen
- Recombinant mouse Osteonectin/SPARC, aa 1-302.
- Target Species
- Mouse
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Enables calcium ion binding activity and extracellular matrix binding activity. Acts upstream of or within several processes, including bone development; cellular response to growth factor stimulus; and pigmentation. Located in extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in cervix carcinoma; endometrial carcinoma; and osteogenesis imperfecta type 17.
- Alternative Names
- ON; BM-40
- Gene ID
- 20692
- UniProt ID
- P07214
Downloads
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IgG Antibodies
Chicken IgY Antibodies
Rabbit Monoclonal Antibodies
ScFv Antibodies
Fab Antibodies
IHC Kits & Antibodies
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0379MZ | Recombinant Mouse Anti-Human Osteonectin Antibody (clone BPO-5032) | RIA, WB, ELISA | Mouse antibody |
HPAB-0303-FY | Human Anti-SPARC Recombinant Antibody (clone Fab6) | ELISA | Humanized IgG |
HPAB-0304-FY | Human Anti-SPARC Recombinant Antibody (clone Fab16) | ELISA | Humanized IgG |
VS3-QX1047 | Mouse Anti-SPARC Recombinant Antibody (clone 664CT7.3.1) | WB | Mouse IgG1 |
VS13-YC1102 | Rabbit Anti-SPARC Recombinant Antibody (VS13-YC1102) | IHC-P | Rabbit IgG |
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