AbPlus™ Anti-Sparc Magnetic Beads (VS-0724-YC635)

Name: AbPlus™ Anti-Sparc Magnetic Beads (VS-0724-YC635)
SKU: VS-0724-YC635
Rating: 5 (1 reviews)

CAT#: VS-0724-YC635

The AbPlus Anti-Sparc Magnetic Beads (VS-0724-YC635) is an innovative affinity resin which is bound with anti-Sparc specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of Sparc protein under native and denaturing conditions.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg

Normal Tissue Figure 3 Colon

Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg

Normal Tissue Figure 6 Placenta

Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC

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Specifications

Applications
Immunoprecipitation, Protein Purification

Matrix
Magnetic bead

Bead Ligand
Anti-Sparc specific antibody

Target
Sparc

Immunogen
Recombinant mouse Osteonectin/SPARC, aa 1-302.

Target Species
Mouse

Bead Capacity
40 mg/mL

Bead size
25 μm

Format
Suspension

Concentration
2 mg/mL

Buffer
PBS, pH 7.4

Preservative
0.1% Sodium azide

Storage
Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

Application Notes
The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

Introduction
Enables calcium ion binding activity and extracellular matrix binding activity. Acts upstream of or within several processes, including bone development; cellular response to growth factor stimulus; and pigmentation. Located in extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in cervix carcinoma; endometrial carcinoma; and osteogenesis imperfecta type 17.

Alternative Names
ON; BM-40

Gene ID
20692

UniProt ID
P07214

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Downloadable Resources

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Datasheet

Datasheet - VS-0724-YC635.pdf

MSDS

MSDS - VS-0724-YC635.pdf

COA

Certificate of Analysis Lookup

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