Anti-Mouse SPARC Immunohistochemistry Kit
CAT#: VS-0525-XY6843
The SPARC IHC kit is optimized for detecting secreted protein acidic and cysteine rich in tissue sections. It includes everything needed for use with both FFPE and frozen tissues, ensuring consistent, high-quality results with minimal background interference and high sensitivity.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
Normal Tissue
Figure 6 Placenta
Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Mouse
- Target
- SPARC
- Primary Antibody
- Rat Anti-SPARC Antibody
- Secondary Antibody
- Goat anti-Rat Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SPARC"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3572z | Mouse Anti-SPARC Recombinant Antibody (clone 41H5) | WB, IP, IF, IHC | Mouse IgG1 |
| HPAB-0267-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 2) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0270-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 1) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0282-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 5) | WB, ELISA | Humanized IgG2a, κ |
| HPAB-0303-FY | Human Anti-SPARC Recombinant Antibody (clone Fab6) | ELISA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0551MZ | Chicken Anti-SPARC Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4435 | Hi-Affi™ Recombinant Rabbit Anti-SPARC Monoclonal Antibody (SI528DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0267-FY-S(P) | Human Anti-SPARC Recombinant Antibody (clone Imml 2); scFv Fragment | WB, ELISA | Humanized scFv |
| HPAB-0270-FY-S(P) | Human Anti-SPARC Recombinant Antibody (clone Imml 1); scFv Fragment | WB, ELISA | Humanized scFv |
| HPAB-0282-FY-S(P) | Human Anti-SPARC Recombinant Antibody (clone Imml 5); scFv Fragment | WB, ELISA | Humanized scFv |
| HPAB-0303-FY-S(P) | Human Anti-SPARC Recombinant Antibody (clone Fab6); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0304-FY-S(P) | Human Anti-SPARC Recombinant Antibody (clone Fab16); scFv Fragment | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0267-FY-F(E) | Human Anti-SPARC Recombinant Antibody (clone Imml 2); Fab Fragment | WB, ELISA | Humanized Fab |
| HPAB-0270-FY-F(E) | Human Anti-SPARC Recombinant Antibody (clone Imml 1); Fab Fragment | WB, ELISA | Humanized Fab |
| HPAB-0282-FY-F(E) | Human Anti-SPARC Recombinant Antibody (clone Imml 5); Fab Fragment | WB, ELISA | Humanized Fab |
| HPAB-0303-FY-F(E) | Human Anti-SPARC Recombinant Antibody (clone Fab6); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0304-FY-F(E) | Human Anti-SPARC Recombinant Antibody (clone Fab16); Fab Fragment | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC635 | AbPlus™ Anti-Sparc Magnetic Beads (VS-0724-YC635) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2157 | Anti-SPARC Immunohistochemistry Kit | IHC | |
| VS-0525-XY6842 | Anti-Human SPARC Immunohistochemistry Kit | IHC |
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