Anti-Mouse SPARC Immunohistochemistry Kit

Name: Anti-Mouse SPARC Immunohistochemistry Kit
SKU: VS-0525-XY6843
Rating: 5 (1 reviews)

CAT#: VS-0525-XY6843

The SPARC IHC kit is optimized for detecting secreted protein acidic and cysteine rich in tissue sections. It includes everything needed for use with both FFPE and frozen tissues, ensuring consistent, high-quality results with minimal background interference and high sensitivity.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg

Normal Tissue Figure 3 Colon

Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg

Normal Tissue Figure 6 Placenta

Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Mouse

Target
SPARC

Primary Antibody
Rat Anti-SPARC Antibody

Secondary Antibody
Goat anti-Rat Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Datasheet

Datasheet - VS-0525-XY6843.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY6843.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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