CytoStream™ Rabbit Anti-SRSF3 Recombinant Antibody (VS13-YC1112)
CAT#: VS13-YC1112
The CytoStream™ Rabbit Anti-SRSF3 Recombinant Antibody is specific for human, mouse and rat SRSF3. SRSF3 encodes a splicing factor for pre-mRNA processing and export, part of the spliceosome, existing in coding and non-coding variants. The anti-SRSF3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56981/168365_B_5_5_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_B_8_5.jpg
Normal Tissue
Figure 5 Cerebellum
Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_B_8_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12986/30654_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112081-SRSF3
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Specifications
- Immunogen
- Recombinant human SRSF3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SRSF3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Serine And Arginine Rich Splicing Factor 3; Splicing Factor, Arginine/Serine-Rich 3; Serine/Arginine-Rich Splicing Factor 3; Pre-MRNA-Splicing Factor SRP20; SRp20; SFRS3; Splicing Factor, Arginine/Serine-Rich, 20-KD; Pre-MRNA Splicing Factor SRp20;
- Gene ID
- 6428
- UniProt ID
- P84103
- Long Name
- Serine And Arginine Rich Splicing Factor 3
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SRSF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1838MZ | Recombinant Mouse Anti-Human SRSF3 Antibody | ELISA, WB | Mouse antibody |
| VS3-WK370 | Rabbit Anti-SRSF3 Recombinant Antibody (clone JE46-44) | WB, IHC, ICC | Rabbit IgG |
| VS3-FY1393 | Recombinant Rabbit Anti-SRSF3 Antibody (clone R01-6G2) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2182 | Anti-SRSF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6910 | Anti-Mouse SRSF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6909 | Anti-Human SRSF3 Immunohistochemistry Kit | IHC |
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