Rabbit Anti-STIP1 Recombinant Antibody (clone R04-3H1) (CAT#: VS3-FY1887)
Clone R04-3H1 is a rabbit IgG recombinant antibody that has a high affinity for human STIP1 protein, a calculated weight of 63 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-3H1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in basal cells of the seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17448/38951_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in basal cells of the seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17448/38951_A_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining:
Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_B_7_5.jpg
(Endothelial cells
Staining:
Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_8_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_8_3.jpg
Figure 5 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_7_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_7_5.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_6_6.jpg
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168439-STIP1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168439-STIP1/subcellular
Specifications
- Immunogen
- Synthetic peptide of human STIP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat STIP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3H1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 63 kDa, while the observed molecular weight stands at 63 kDa.
- Related Disease
- Microcephaly 7, Primary, Autosomal Recessive and Retinitis Pigmentosa 61
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
- Gene ID
- 10963
- UniProt ID
- P31948
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys10, Lys13, Lys32, Lys68, Lys109, Lys123, Lys100, Lys246, Lys284, Lys325, Lys434, Lys442, Lys446, Lys462, and Lys513 (NX_P31948 [NX_P31948-1])
Modification sites at PhosphoSitePlus (P31948)
Glycosylation from GlyGen (P31948) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_001269581.1; NP_001269582.1; NP_006810.1
- Function
- Acts as a co-chaperone for HSP90AA1.
Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2513z | Mouse Anti-STIP1 Recombinant Antibody (clone 38A1) | WB, ELISA, ICC, IF, IHC | Mouse IgG |
| MOB-1963CT | Recombinant Mouse anti-Human STIP1 Monoclonal antibody (EML1213) | IHC-P, IP, WB | |
| MRO-1447-CN | Recombinant Rabbit Anti-STIP1 Monoclonal Antibody (JB95-38) | WB, IHC, FC, IP, IF | Rabbit IgG |
| ZG-0586U | Rabbit Anti-STIP1 Recombinant Antibody (clone 3E4) | ELISA, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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