Rabbit Anti-STIP1 Recombinant Antibody (clone R04-3H1) (CAT#: VS3-FY1887)
Clone R04-3H1 is a rabbit IgG recombinant antibody that has a high affinity for human STIP1 protein, a calculated weight of 63 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-3H1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in basal cells of the seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17448/38951_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in basal cells of the seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17448/38951_A_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining:
Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_B_7_5.jpg
(Endothelial cells
Staining:
Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_8_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_8_3.jpg
Figure 5 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_7_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_7_5.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_6_6.jpg
(Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39291/108431_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168439-STIP1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168439-STIP1/subcellular
Specifications
- Immunogen
- Synthetic peptide of human STIP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat STIP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3H1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 63 kDa, while the observed molecular weight stands at 63 kDa.
- Related Disease
- Microcephaly 7, Primary, Autosomal Recessive and Retinitis Pigmentosa 61
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
- Gene ID
- 10963
- UniProt ID
- P31948
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys10, Lys13, Lys32, Lys68, Lys109, Lys123, Lys100, Lys246, Lys284, Lys325, Lys434, Lys442, Lys446, Lys462, and Lys513 (NX_P31948 [NX_P31948-1])
Modification sites at PhosphoSitePlus (P31948)
Glycosylation from GlyGen (P31948) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_001269581.1; NP_001269582.1; NP_006810.1
- Function
- Acts as a co-chaperone for HSP90AA1.
Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2513z | Mouse Anti-STIP1 Recombinant Antibody (clone 38A1) | WB, ELISA, ICC, IF, IHC | Mouse IgG |
| MOB-1963CT | Recombinant Mouse anti-Human STIP1 Monoclonal antibody (EML1213) | IHC-P, IP, WB | |
| MRO-1447-CN | Recombinant Rabbit Anti-STIP1 Monoclonal Antibody (JB95-38) | WB, IHC, FC, IP, IF | Rabbit IgG |
| ZG-0586U | Rabbit Anti-STIP1 Recombinant Antibody (clone 3E4) | ELISA, WB | Rabbit IgG |
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