Mouse Anti-SUCLG2 Recombinant Antibody (clone 2506W53)
CAT#: VS-0625-FY90
This Mouse IgG1 kappa monoclonal antibody, clone 2506W53, specifically targets human SUCLG2. It was raised against an internal region of human SUCLG2 (amino acids 121-229). With an observed antigen size of about 37 kDa (calculated 51.58 kDa), this antibody is validated for Western Blot, Immunoprecipitation, Immunofluorescence, and ELISA, offering versatile detection capabilities for SUCLG2.
Specifications
- Immunogen
- Antibody raised against human SUCLG2, targeting amino acids 121-229 within an internal region.
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Species Reactivity
- Human
- Clone
- 2506W53
- Applications
- Western Blot, Immunoprecipitation, Immunofluorescence, ELISA
- Related Disease
- Mitochondrial Dna Depletion Syndrome 9 and Pyruvate Dehydrogenase E1-Alpha Deficiency
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- G-SCS; GBETA; GTPSCS
- Gene ID
- 8801
- UniProt ID
- Q96I99
- Long Name
- succinate-CoA ligase GDP-forming subunit beta
- Sequence Similarities
- Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Ubiquitination at Lys118
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan
- Protein Refseq
- XP_016862909.1; XP_047305096.1; XP_054204242.1; XP_054204243.1; NP_001171070.1
- Function
- GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA.
The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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