Rabbit Anti-SYNCRIP Recombinant Antibody (clone R06-8G9)
CAT#: VS3-FY1740
This R06-8G9 recombinant monoclonal antibody is designed to target human SYNCRIP. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The hnRNP Q antibody is recommended for detection of target protein by western blot analysis of hnRNP Q in K562 lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/996_H9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_B_8_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_B_3_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41275/132755_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135316-SYNCRIP/subcellular
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❯
WB
Figure 1 Anti-SYNCRIP Antibody (VS3-FY1740) in WB.
The hnRNP Q expression in K562 lysates were detected using the anti-hnRNP Q by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human hnRNP Q
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SYNCRIP
- Species Reactivity
- Human, Mouse
- Clone
- R06-8G9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Non-Specific Syndromic Intellectual Disability and Spinal Muscular Atrophy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- PP68; NSAP1; GRYRBP; HNRNPQ; HNRPQ1; GRY-RBP; hnRNP-Q
- Gene ID
- 10492
- UniProt ID
- O60506
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus, Spliceosome
- Post Translation Modifications
- Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity).
- Protein Refseq
- XP_005248692.1; XP_005248694.1; XP_016865665.1; XP_016865666.1; XP_016865667.1
- Function
- Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SYNCRIP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3479 | Hi-Affi™ Recombinant Rabbit Anti-SYNCRIP Monoclonal Antibody (DS3479AB) | IHC-P, ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0762-CN | Recombinant Rabbit Anti-SYNCRIP Monoclonal Antibody (JG35-72) | WB, IF, IHC | Rabbit IgG |
| ZG-0069U | Rabbit Anti-SYNCRIP Recombinant Antibody (clone 9C7) | ELISA, IHC, IF | Rabbit IgG |
| VS1-YJ104 | Mouse Anti-SYNCRIP Recombinant Antibody (clone I8E4) | ELISA, IF, WB, FC, IP | Mouse IgG1, κ |
| VS3-WK454 | Rabbit Anti-SYNCRIP Recombinant Antibody (clone JG35-72) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2235 | Anti-SYNCRIP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7048 | Anti-Human SYNCRIP Immunohistochemistry Kit | IHC |
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