Mouse Anti-SYNE1 Recombinant Antibody (clone MANNES1A(7A12))
CAT#: VS1-YJ140
This product is a mouse antibody that recognizes SYNE1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic and nuclear membranous positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/247_D1_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/44051_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/44051_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/44051_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/44051_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19113/44051_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-2 OS, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131018-SYNE1
❮
❯
Specifications
- Immunogen
- Recombinant full length Nesprin 1(Human)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SYNE1
- Species Reactivity
- Mouse, Rat, Rabbit, Human
- Clone
- MANNES1A(7A12)
- Applications
- IB, IF, WB
Product Property
- Purification
- Protein G purified
- Concentration
- See the COA
- Buffer
- See the COA
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunoblotting, Immunofluorescence, Western Blot.
Target
- Alternative Names
- 8B; AMC3; AMCM; CPG2; ARCA1; EDMD4; KASH1; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2; nesprin-1; CPG2 full length KASH domain-containing protein 1 enaptin myocyte nuclear envelope protein 1 nesprin 1 spectrin repeat containing, nuclear envelope 1 synaptic nuclear envelope protein 1 synaptic nuclei expressed gene 1
- Gene ID
- 23345
- UniProt ID
- Q8NF91
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RESOURCES
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SYNE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1238z | Mouse Anti-SYNE1 Recombinant Antibody (clone 25H2) | WB, IP, ELISA | Mouse IgG3, κ |
| MOB-1182CT | Recombinant Mouse anti-Human SYNE1 Monoclonal antibody (EML2187) | WB | |
| MRO-1074-CN | Recombinant Rabbit Anti-SYNE1 Monoclonal Antibody (JB35-26) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK285 | Rabbit Anti-SYNE1 Recombinant Antibody (clone JB35-26) | WB, ELISA | Rabbit IgG |
| VS3-FY1419 | Recombinant Rabbit Anti-SYNE1 Antibody (clone R07-4E7) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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