Rabbit Anti-TBK1 Recombinant Antibody (clone R01-1G3) (CAT#: VS3-FY1630)

• Immunogen
• Synthetic peptide of human TBK1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat, Hamster TBK1

• Species Reactivity
• Human, Mouse, Rat, Hamster

• Clone
• R01-1G3

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 84 kDa, while the observed molecular weight stands at 84 kDa.

• Related Disease
• Encephalopathy, Acute, Infection-Induced 8 and Frontotemporal Dementia And/Or Amyotrophic Lateral Sclerosis 4

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• TANK Binding Kinase 1; NF-Kappa-B-Activating Kinase; TANK-Binding Kinase 1; NAK; T2K; Serine/Threonine-Protein Kinase TBK1

• Gene ID
• 29110

• UniProt ID
• Q9UHD2

• Sequence Similarities
• Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Autophosphorylation at Ser-172 activates the kinase, and is an essential step for virus-triggered signaling. Phosphorylated by IKBKB/IKKB at Ser-172. Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401. Dephosphorylated at Ser-172 by PPM1B and this negatively regulates its role in mediating antiviral response., 'Lys-63'-linked polyubiquitination by MIB1 after RNA virus infection, or by NRDP1 after LPS stimulation at Lys-30 and Lys-401, participates in kinase activation. 'Lys-48'-linked polyubiquitination at Lys-670 by DTX4 leads to proteasomal degradation. 'Lys-48'-linked polyubiquitination by TRAIP also leads to proteasomal degradation. 'Lys-63'-linked polyubiquitination by RNF128 at Lys-30 and Lys-401 leads to the activation of antiviral responses., (Microbial infection) Interaction with SARS-CoV-2 M protein induces 'Lys-48'-linked ubiquitination which leads to proteasomal degradation.

• Protein Refseq
• NP_037386.1

• Function
• Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents.
  Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X.
  This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB.
  In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli.
  Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody