Rabbit Anti-TBK1 Recombinant Antibody (clone R01-2C4) (CAT#: VS3-XY3518)
This is a recombinant rabbit antibody that recognizes human TBK1 (TANK binding kinase 1), which acts both as a positive and negative regulator of the mTORC1 complex. The antibody is capable of recognizing human, mouse and rat TBK1. It has been repored for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/605_C2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/605_C2_2_selected.jpg
Figure 2 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60211/1422_G11_1_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60211/1422_G11_1_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_A_8_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45797/104323_A_5_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183735-TBK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183735-TBK1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TBK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-2C4
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Encephalopathy, Acute, Infection-Induced 8; Frontotemporal Dementia; Amyotrophic Lateral Sclerosis 4
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- NAK; T2K; IIAE8; FTDALS4
- Gene ID
- 29110
- UniProt ID
- Q9UHD2
- Long Name
- TANK binding kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Autophosphorylation at Ser-172 activates the kinase, and is an essential step for virus-triggered signaling. Phosphorylated by IKBKB/IKKB at Ser-172. Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401. Dephosphorylated at Ser-172 by PPM1B and this negatively regulates its role in mediating antiviral response.
- Protein Refseq
- NP_037386.1
- Function
- Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents.
Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X.
This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TLR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2235MZ | Recombinant Mouse Anti-Human TBK1 Antibody (clone 209B530) | FC, IP, WB | Mouse antibody |
| VS4-CJ131 | Recombinant Rabbit Anti-TBK1 Antibody (clone 14D10) | ELISA, IHC, FC | Rabbit IgG |
| VS3-FY1429 | Recombinant Rabbit Anti-TBK1 Antibody (clone R05-2H7) | WB | Rabbit IgG |
| VS3-FY1630 | Rabbit Anti-TBK1 Recombinant Antibody (clone R01-1G3) | WB | Rabbit IgG |
| VS3-WK2066 | Rabbit Anti-TBK1 (pS172) Recombinant Antibody (clone R92-5Y-2) | WB, IP | Rabbit IgG |
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