Mouse Anti-TBP Recombinant Antibody (clone 4H2) (CAT#: VS3-CJ762)

• Immunogen
• Recombinant protein

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human, Mouse, Rat endogenous levels of TBP/TATA Binding Protein

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 4H2

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:2000).

• Alternative Names
• HDL4; TBP1; GTF2D; SCA17; TFIID; GTF2D1

• Gene ID
• 6908

• UniProt ID
• P20226

• Sequence Similarities
• Belongs to the TBP family.

• Cellular Localization
• Nucleus

• Protein Refseq
• NP_001165556.1; NP_003185.1

• Function
• The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
  TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612).
  The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:27007846).
  The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473).
  TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed:33795473).
  TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:9836642).
  During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed:33795473).
  Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071).
  Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed:15970593).
  The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed:15970593).
  SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed:15970593).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS