Mouse Anti-TIMP2 Recombinant Antibody (VS3-FY2897)
CAT#: VS3-FY2897
This mouse anti-TIMP2 recombinant monoclonal antibody has been genetically engineered to specifically target the human, mouse, rat TIMP2 protein. The calculated molecular weight of the protein is about 24.4 kDa. This monoclonal antibody proves valuable in disease research related to Conjunctivochalasis and Fibrosarcoma. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Immunocytochemistry (1/10-1/25) and Flow cytometry (1/10-1/25).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows cytoplasmic and extracellular positivity in cells of tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/25187_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/24842_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/24842_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/24842_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/24842_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10203/24842_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ hTERT+, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000035862-TIMP2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat TIMP2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 24.4 kDa.
- Related Disease
- Conjunctivochalasis and Fibrosarcoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/10-1/25
Flow cytometry: 1/10-1/25
Target
- Alternative Names
- TIMP Metallopeptidase Inhibitor 2; Tissue Inhibitor Of Metalloproteinases 2; CSC-21K; Tissue Inhibitor Of Metalloproteinase 2; Testicular Secretory Protein Li 57; Metalloproteinase Inhibitor 2; TIMP-2; DDC8
- Gene ID
- 7077
- UniProt ID
- P16035
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- The activity of TIMP2 is dependent on the presence of disulfide bonds. (P16035-TIMP2_HUMAN)
Modification sites at PhosphoSitePlus (P16035)
Modification sites at neXtProt
- Function
- Belongs to the protease inhibitor I35 (TIMP) family.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TIMP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1841z | Mouse Anti-TIMP2 Recombinant Antibody (clone 9C12) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0860CT | Recombinant Mouse anti-Human TIMP2 Monoclonal antibody (U3-202) | IP, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3576 | Rabbit Anti-TIMP2 Recombinant Antibody (clone DS3576AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1498-CN | Rabbit Anti-TIMP2 Recombinant Antibody (clone JM87-10) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0715F | Mouse Anti-TIMP2 Recombinant Antibody (clone 3A4) | WB, ELISA, WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ683 | Mouse Anti-TIMP2 Recombinant Antibody (VS3-CJ683) | WB, ICC, IHC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2295 | Anti-TIMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7271 | Anti-Mouse TIMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7270 | Anti-Human TIMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7272 | Anti-Canine TIMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7273 | Anti-Bovine TIMP2 Immunohistochemistry Kit | IHC |
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