Mouse Anti-TNNI3 Monoclonal Antibody (VS7-0425-WR1031) (CAT#: VS7-0425-WR1031)
This product is a mouse IgG antibody that binds to human TNNI3. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Figure 2 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Figure 4 Testis
(Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
(Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Figure 5 Heart muscle
(Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
(Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
Specifications
- Immunogen
- Recombinant human TNNI3 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TNNI3
- Species Reactivity
- Human
- Molecule Class
- Troponin I
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cardiomyopathy, familial hypertrophic, 7 (CMH7); Cardiomyopathy, familial restrictive 1 (RCM1); Cardiomyopathy, dilated, 2A (CMD2A); Cardiomyopathy, dilated, 1FF (CMD1FF)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TNNI3; troponin I type 3 (cardiac); CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
- Gene ID
- 7137
- UniProt ID
- P19429
- Long Name
- Troponin I3, Cardiac Type
- Cellular Localization
- cardiac myofibril; cytosol; sarcomere
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000354.4
- Function
- Actin-binding; Muscle protein
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1096 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| DrMAB-1099 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| FAMAB-0154-CN-S(P) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); scFv Fragment | ELISA | Rabbit scFv |
| HPAB-0942-FY-F(E) | Mouse Anti-TNNI3 Recombinant Antibody Fab Fragment (HPAB-0942-FY-F(E)) | FuncS, ELISA | Mouse Fab |
| HPAB-0497-YJ-S(P) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); scFv Fragment | ELISA | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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