Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905)
CAT#: VS3-FY2905
The anti-TOP2A antibody is a mouse recombinant monoclonal antibody that has been specifically designed to target the human, rat TOP2A protein. The calculated molecular weight of the protein is about 174.4 kDa. The monoclonal antibody can be used in the area of Malignant Peripheral Nerve Sheath Tumor and Neuroblastoma.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Normal Tissue
Figure 2 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Rat TOP2A
- Species Reactivity
- Human, Rat
- Applications
- Western Blot, Immunocytochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 174.4 kDa.
- Related Disease
- Malignant Peripheral Nerve Sheath Tumor and Neuroblastoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunocytochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- DNA Topoisomerase II Alpha; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; EC 5.99.1.3; TOP2; DNA Topoisomerase (ATP-Hydrolyzing)
- Gene ID
- 7153
- UniProt ID
- P11388
- Sequence Similarities
- Belongs to the type II topoisomerase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Protein Refseq
- NP_001058.2
- Function
- Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TOP2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| ZG-0598U | Rabbit Anti-TOP2A Recombinant Antibody (clone 5B10) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ593 | Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593) | WB, IF, IHC | Mouse IgG2b |
| VS3-CJ594 | Rabbit Anti-TOP2A Recombinant Antibody (VS3-CJ594) | WB, IHC, IP | Rabbit IgG |
| VS3-FY1471 | Recombinant Mouse Anti-TOP2A Antibody (clone 1D2-2H6-7D1) | IHC-P | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7427 | Anti-TOP2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY7428 | Anti-Mouse TOP2A Immunohistochemistry Kit | IHC |
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