Rabbit Anti-TPMT Recombinant Antibody (clone R02-0G7) (CAT#: VS3-FY2094)
This rabbit recombinant monoclonal antibody can be used in western blot analysis on TPMT in HeLa, A549, and HL-60 lysates, and perform immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma. It demonstrates high specificity in binding to human TPMT. This R02-0G7 antibody is validated for western blot analysis of TPMT in Hela, A549, HL-60 lysates and immunohistochemistry analysis of paraffin-embedded human Cholangiocarcinoma.
Specific Inquiry
WB
Figure 1 Anti-TPMT Antibody (VS3-FY2094) in WB.
The TPMT expression in Hela, A549, HL-60 lysates were detected using the anti-TPMT by western blot assay.
IHC
Figure 2 Anti-TPMT Antibody (VS3-FY2094) in IHC.
Immunohistochemistry staining of paraffin-embedded human Cholangiocarcinoma using the anti-TPMT antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human TPMT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPMT
- Species Reactivity
- Human
- Clone
- R02-0G7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28 kDa.
- Related Disease
- Thiopurines, Poor Metabolism Of, 1 and Thiopurine S Methyltranferase Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thiopurine S-Methyltransferase; EC 2.1.1.67; S-Adenosyl-L-Methionine:Thiopurine S-Methyltransferase; Thiopurine Methyltransferase; TPMTD
- Gene ID
- 7172
- UniProt ID
- P51580
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. TPMT family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys37 (NX_P51580 [NX_P51580-1])
Modification sites at PhosphoSitePlus (P51580)
Glycosylation from GlyGen (P51580) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047275245.1; XP_047275246.1; XP_054212273.1; XP_054212274.1; NP_000358.1
- Function
- TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3613z | Mouse Anti-TPMT Recombinant Antibody (clone 15H3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1, κ |
| MOB-1815MZ | Recombinant Mouse Anti-Human TPMT Antibody | ICC/IF, IHC-P, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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