Rabbit Anti-TPMT Recombinant Antibody (clone R02-0G7)
CAT#: VS3-FY2094
This rabbit recombinant monoclonal antibody can be used in western blot analysis on TPMT in HeLa, A549, and HL-60 lysates, and perform immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma. It demonstrates high specificity in binding to human TPMT. This R02-0G7 antibody is validated for western blot analysis of TPMT in Hela, A549, HL-60 lysates and immunohistochemistry analysis of paraffin-embedded human Cholangiocarcinoma.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Thyroid gland
High expression in thyroid gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19851/46318_B_3_5_val_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62364/168977_A_8_5_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19851/46318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19851/46318_A_7_4.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137364-TPMT
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WB
Figure 1 Anti-TPMT Antibody (VS3-FY2094) in WB.
The TPMT expression in Hela, A549, HL-60 lysates were detected using the anti-TPMT by western blot assay.
IHC
Figure 2 Anti-TPMT Antibody (VS3-FY2094) in IHC.
Immunohistochemistry staining of paraffin-embedded human Cholangiocarcinoma using the anti-TPMT antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human TPMT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPMT
- Species Reactivity
- Human
- Clone
- R02-0G7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28 kDa.
- Related Disease
- Thiopurines, Poor Metabolism Of, 1 and Thiopurine S Methyltranferase Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thiopurine S-Methyltransferase; EC 2.1.1.67; S-Adenosyl-L-Methionine:Thiopurine S-Methyltransferase; Thiopurine Methyltransferase; TPMTD
- Gene ID
- 7172
- UniProt ID
- P51580
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. TPMT family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys37 (NX_P51580 [NX_P51580-1])
Modification sites at PhosphoSitePlus (P51580)
Glycosylation from GlyGen (P51580) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047275245.1; XP_047275246.1; XP_054212273.1; XP_054212274.1; NP_000358.1
- Function
- TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TPMT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3613z | Mouse Anti-TPMT Recombinant Antibody (clone 15H3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1, κ |
| MOB-1815MZ | Recombinant Mouse Anti-Human TPMT Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3657 | Rabbit Anti-TPMT Recombinant Antibody (clone DS3657AB) | WB, IHC-P, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7467 | Anti-TPMT Immunohistochemistry Kit | IHC | |
| VS-0525-XY7468 | Anti-Mouse TPMT Immunohistochemistry Kit | IHC |
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