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    Mouse Anti-TRIM25 Recombinant Antibody (clone 5B5B10) (CAT#: VS7-0425-WR1048)

    This product is a mouse IgG2b antibody that binds to human TRIM25. The antibody 5B5B10 recognizes specific amino acid domain of human TRIM25 (AA: 211-360). It can be used for WB, IHC, ICC, and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

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    • Purity:

    • Formats:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/8_B5_1_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human stomach
    Figure 2 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/16356_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/16356_A_8_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/16356_A_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/16356_A_6_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5909/16356_A_8_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121060-TRIM25

    Specifications

    • Immunogen
    • Recombinant human TRIM25 (AA: 211-360) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2b
    • Specificity
    • Human TRIM25
    • Species Reactivity
    • Human
    • Clone
    • 5B5B10
    • Molecule Class
    • Trim Protein
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:50 - 1:250
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • EFP; Z147; RNF147; ZNF147
    • Long Name
    • Tripartite motif containing 25
    • Cellular Localization
    • Cytoplasm; Nucleus
    • Post Translation Modifications
    • Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • Ligase; Transferase

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "TRIM25"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOB-0864CT Recombinant Mouse anti-Human TRIM25 Monoclonal antibody (5C3) ELISA, WB
    VS7-0425-WR1047 Mouse Anti-TRIM25 Recombinant Antibody (clone 5B5B12) WB, IHC, ICC, FC Mouse IgG2b

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