Rabbit Anti-TRMT2A Recombinant Antibody (clone R03-3H4) (CAT#: VS3-FY1607)
The R03-3H4 recombinant monoclonal antibody demonstrates high specificity in binding to human TRMT2A. The antibody is recommended for detection of TRMT2A protein by WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The TRMT2A antibody has been tested by western blot analysis of HTF9C/TRMT2A in K562, Hela lysates and immunocytochemistry analysis of HTF9C/TRMT2A (green) in Hela using HTF9C/TRMT2A antibody.
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WB
Figure 1 Anti-TRMT2A Antibody (VS3-FY1607) in WB.
The HTF9C/TRMT2A expression in K562, Hela lysates were detected using the anti-TRMT2A by western blot assay.
ICC
Figure 2 Anti-TRMT2A Antibody (VS3-FY1607) in ICC.
Immunocytochemistry staining of HTF9C/TRMT2A (green) in Hela using the anti-HTF9C/TRMT2A antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human HTF9C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TRMT2A
- Species Reactivity
- Human
- Clone
- R03-3H4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 69 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9c Protein; HTF9C; TRNA Methyltransferase 2 Homolog A (S. Cerevisiae); TRNA (Uracil-5-)-Methyltransferase Homolog A; TRM2 TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9C; EC 2.1.1.-
- Gene ID
- 27037
- UniProt ID
- Q8IZ69
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001244923.1; NP_001317968.1; NP_073564.3; NP_892029.2
- Function
- S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs.
Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs.
Also able to mediate the formation of 5-methyl-uridine in some mRNAs.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3693 | Rabbit Anti-TRMT2A Recombinant Antibody (clone DS3693AB) | WB, IHC, ICC | Rabbit IgG |
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