Rabbit Anti-TRMT2A Recombinant Antibody (clone R03-3H4) (CAT#: VS3-FY1607)
The R03-3H4 recombinant monoclonal antibody demonstrates high specificity in binding to human TRMT2A. The antibody is recommended for detection of TRMT2A protein by WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The TRMT2A antibody has been tested by western blot analysis of HTF9C/TRMT2A in K562, Hela lysates and immunocytochemistry analysis of HTF9C/TRMT2A (green) in Hela using HTF9C/TRMT2A antibody.
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WB
Figure 1 Anti-TRMT2A Antibody (VS3-FY1607) in WB.
The HTF9C/TRMT2A expression in K562, Hela lysates were detected using the anti-TRMT2A by western blot assay.
ICC
Figure 2 Anti-TRMT2A Antibody (VS3-FY1607) in ICC.
Immunocytochemistry staining of HTF9C/TRMT2A (green) in Hela using the anti-HTF9C/TRMT2A antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human HTF9C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TRMT2A
- Species Reactivity
- Human
- Clone
- R03-3H4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 69 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9c Protein; HTF9C; TRNA Methyltransferase 2 Homolog A (S. Cerevisiae); TRNA (Uracil-5-)-Methyltransferase Homolog A; TRM2 TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9C; EC 2.1.1.-
- Gene ID
- 27037
- UniProt ID
- Q8IZ69
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001244923.1; NP_001317968.1; NP_073564.3; NP_892029.2
- Function
- S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs.
Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs.
Also able to mediate the formation of 5-methyl-uridine in some mRNAs.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3693 | Rabbit Anti-TRMT2A Recombinant Antibody (clone DS3693AB) | WB, IHC, ICC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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