Rabbit Anti-TRMT2A Recombinant Antibody (clone R03-3H4)
CAT#: VS3-FY1607
The R03-3H4 recombinant monoclonal antibody demonstrates high specificity in binding to human TRMT2A. The antibody is recommended for detection of TRMT2A protein by WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The TRMT2A antibody has been tested by western blot analysis of HTF9C/TRMT2A in K562, Hela lysates and immunocytochemistry analysis of HTF9C/TRMT2A (green) in Hela using HTF9C/TRMT2A antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1077/2704_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (K-562)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099899-TRMT2A
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WB
Figure 1 Anti-TRMT2A Antibody (VS3-FY1607) in WB.
The HTF9C/TRMT2A expression in K562, Hela lysates were detected using the anti-TRMT2A by western blot assay.
ICC
Figure 2 Anti-TRMT2A Antibody (VS3-FY1607) in ICC.
Immunocytochemistry staining of HTF9C/TRMT2A (green) in Hela using the anti-HTF9C/TRMT2A antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human HTF9C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TRMT2A
- Species Reactivity
- Human
- Clone
- R03-3H4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 69 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9c Protein; HTF9C; TRNA Methyltransferase 2 Homolog A (S. Cerevisiae); TRNA (Uracil-5-)-Methyltransferase Homolog A; TRM2 TRNA Methyltransferase 2 Homolog A; HpaII Tiny Fragments Locus 9C; EC 2.1.1.-
- Gene ID
- 27037
- UniProt ID
- Q8IZ69
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001244923.1; NP_001317968.1; NP_073564.3; NP_892029.2
- Function
- S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs.
Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs.
Also able to mediate the formation of 5-methyl-uridine in some mRNAs.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TRMT2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3693 | Rabbit Anti-TRMT2A Recombinant Antibody (clone DS3693AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7547 | Anti-TRMT2A Immunohistochemistry Kit | IHC |
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