Anti-TSC2 Immunohistochemistry Kit (CAT#: VS-0325-XY2377)
The TSC2 IHC Kit provides a robust platform for mTOR pathway and cancer metabolism studies, featuring pre-validated antibodies optimized for FFPE and frozen tumor samples. Its user-friendly workflow minimizes steps, from antigen retrieval to counterstaining, while dual tissue compatibility ensures flexible analysis of TSC2's role in cell growth control. Achieve reproducible results for drug development and diagnostic applications.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/if_selected.jpg
Figure 2 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49679/if_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49679/if_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2225/710_C8_6_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2225/710_C8_6_selected.jpg
Figure 4 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_7_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_7_5_selected.jpg
Figure 5 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/335_A8_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/335_A8_1_blue_red_green.jpg
Figure 6 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_8_5.jpg
Figure 7 Colon
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_9_3.jpg
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_9_3.jpg
Figure 8 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_4.jpg
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_4.jpg
Figure 9 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_5.jpg
Figure 10 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_4_6.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000103197-TSC2
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000103197-TSC2
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103197-TSC2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103197-TSC2
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Rat
- Target
- TSC2
- Primary Antibody
- Mouse Anti-TSC2 Monoclonal Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
Related Resources
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Insulin Resistance
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-636 | Human Anti-TSC2 Recombinant Antibody (clone D542) | ELISA, IP-MS | Human IgG |
| MRO-1538-CN | Rabbit Anti-TSC2 Recombinant Antibody (clone CBACN-564) | WB, IHC, FC | Rabbit IgG |
| MRO-2245-CN | Rabbit Anti-TSC2 Polyclonal Antibody (MRO-2245-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-CJ997 | Rabbit Anti-TSC2 Recombinant Antibody (VS3-CJ997) | WB, IHC, FC | Rabbit IgG |
| VS3-FY1490 | Recombinant Rabbit Anti-TSC2 Antibody (clone R06-0D3) | WB, IHC-P, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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