Recombinant Rabbit Anti-TSC2 Antibody (clone R06-0D3)
CAT#: VS3-FY1490
This product is a recombinant rabbit antibody that recognizes TSC2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R06-0D3 is specific for human TSC2. The antigen is a synthetic peptide from human potato.
Tested Data
Gene Expression
IHC-P
Figure 1. Recombinant Rabbit Anti-TSC2 Antibody (clone R06-0D3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using Tuberin Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49679/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2225/710_C8_6_selected.jpg
Subcellular Location
Figure 4 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_7_5_selected.jpg
Subcellular Location
Figure 5 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/335_A8_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103197-TSC2
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Specifications
- Immunogen
- A synthetic peptide from human potato.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TSC2
- Species Reactivity
- Human
- Clone
- R06-0D3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 201 kDa; Observed MW: 201 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- TSC Complex Subunit 2; Protein Phosphatase 1, Regulatory Subunit 160; Tuberous Sclerosis 2 Protein; TSC4; Tuberous Sclerosis 2; PPP1R160; Tuberin; LAM
- Gene ID
- 7249
- UniProt ID
- P49815
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.
Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.
- Protein Refseq
- NP_000539.2; NP_001070651.1; NP_001107854.1
- Function
- In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400).
Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:15340059).
May also play a role in microtubule-mediated protein transport (By similarity).
Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).
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Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TSC2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-636 | Human Anti-TSC2 Recombinant Antibody (clone D542) | ELISA, IP-MS | Human IgG |
| MRO-1538-CN | Rabbit Anti-TSC2 Recombinant Antibody (clone CBACN-564) | WB, IHC, FC | Rabbit IgG |
| MRO-2245-CN | Rabbit Anti-TSC2 Polyclonal Antibody (MRO-2245-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-CJ997 | Rabbit Anti-TSC2 Recombinant Antibody (VS3-CJ997) | WB, IHC, FC | Rabbit IgG |
| VS3-FY2098 | Rabbit Anti-TSC2 Recombinant Antibody (clone R04-1I3) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3701 | Rabbit Anti-TSC2 Recombinant Antibody (clone DS3701AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2377 | Anti-TSC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7565 | Anti-Mouse TSC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7564 | Anti-Human TSC2 Immunohistochemistry Kit | IHC |
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