Rabbit Anti-TXN Recombinant Antibody (clone R07-4H5)
CAT#: VS3-FY1622
This clone R07-4H5 is a rabbit recombinant monoclonal antibody that effectively detects human TXN. The antibody is recommended for detection of TXN protein by WB, IHC-F, IHC-P, ICC/IF, IP. The dilution ratio that is advised for optimal results is as follows: western blot (dilution 1/1000-1/10000), immunohistochemistry (dilution 1/50-1/200), immunofluorescence (dilution 1/100-1/500), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/751_E4_1_selected.jpg
Normal Tissue
Figure 2 Esophagus
Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47478/139514_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136810-TXN/subcellular
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WB
Figure 1 Anti-TXN Antibody (VS3-FY1622) in WB.
The Thioredoxin expression in Jurkat, Hela lysates were detected using the anti-Thioredoxin (TXN) by western blot assay.
ICC
Figure 2 Anti-TXN Antibody (VS3-FY1622) in ICC.
Immunocytochemistry staining of Thioredoxin (green) in Hela using the anti-Thioredoxin antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human thioredoxin/TRX
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TXN
- Species Reactivity
- Human
- Clone
- R07-4H5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 12 kDa, while the observed molecular weight stands at 12 kDa.
- Related Disease
- Retinal Ischemia and Malignant Pleural Mesothelioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/1000-1/10000
Immunohistochemistry: 1/50-1/200
Immunofluorescence: 1/100-1/500
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thioredoxin; Surface-Associated Sulphydryl Protein; ATL-Derived Factor; SASP; TRX1; TRDX
- Gene ID
- 7295
- UniProt ID
- P10599
- Sequence Similarities
- Belongs to the thioredoxin family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins., In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
- Protein Refseq
- NP_001231867.1; NP_003320.2
- Function
- Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TXN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-F(E) | Recombinant Anti-human TXN Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| MHH-1017-F(E) | Recombinant Human Anti-human TXN Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-S(P) | Recombinant Anti-human TXN Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-1017-S(P) | Recombinant Human Anti-human TXN Antibody scFv Fragment | WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3728 | Rabbit Anti-TXN Recombinant Antibody (clone DS3728AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-140R | Mouse Anti-TXN Recombinant Antibody (ZG-140R) | WB, ELISA | Mouse IgG |
| ZG-0613U | Rabbit Anti-TXN Recombinant Antibody (clone 3G7) | ELISA, WB | Rabbit IgG |
| ZG-0614U | Rabbit Anti-TXN Recombinant Antibody (clone 3H8) | ELISA, WB | Rabbit IgG |
| VS3-CJ566 | Mouse Anti-TXN Recombinant Antibody (VS3-CJ566) | WB | Mouse IgG1, κ |
| VS3-FY1500 | Recombinant Rabbit Anti-TXN Antibody (clone R07-4A4) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY268 | AbPlus™ Anti-TXN Magnetic Beads (JM10-019) | IP, Protein Purification | |
| VS-0724-YC1241 | AbPlus™ Anti-TXN Magnetic Beads (VS-0724-YC1241) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2398 | Anti-TXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY7644 | Anti-Human TXN Immunohistochemistry Kit | IHC |
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