Rabbit Anti-TXN Recombinant Antibody (clone R07-4H5) (CAT#: VS3-FY1622)
This clone R07-4H5 is a rabbit recombinant monoclonal antibody that effectively detects human TXN. The antibody is recommended for detection of TXN protein by WB, IHC-F, IHC-P, ICC/IF, IP. The dilution ratio that is advised for optimal results is as follows: western blot (dilution 1/1000-1/10000), immunohistochemistry (dilution 1/50-1/200), immunofluorescence (dilution 1/100-1/500), immunoprecipitation (dilution 1/20).
Specific Inquiry
WB
Figure 1 Anti-TXN Antibody (VS3-FY1622) in WB.
The Thioredoxin expression in Jurkat, Hela lysates were detected using the anti-Thioredoxin (TXN) by western blot assay.
ICC
Figure 2 Anti-TXN Antibody (VS3-FY1622) in ICC.
Immunocytochemistry staining of Thioredoxin (green) in Hela using the anti-Thioredoxin antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human thioredoxin/TRX
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TXN
- Species Reactivity
- Human
- Clone
- R07-4H5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 12 kDa, while the observed molecular weight stands at 12 kDa.
- Related Disease
- Retinal Ischemia and Malignant Pleural Mesothelioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/1000-1/10000
Immunohistochemistry: 1/50-1/200
Immunofluorescence: 1/100-1/500
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thioredoxin; Surface-Associated Sulphydryl Protein; ATL-Derived Factor; SASP; TRX1; TRDX
- Gene ID
- 7295
- UniProt ID
- P10599
- Sequence Similarities
- Belongs to the thioredoxin family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins., In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
- Protein Refseq
- NP_001231867.1; NP_003320.2
- Function
- Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TXN"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017 | Recombinant Anti-human TXN Antibody | ELISA, WB, FC, FuncS | IgG |
| MOB-1167MZ | Recombinant Mouse Anti-Human TXN Antibody (clone 3A1) | WB | Mouse antibody |
| VS3-CJ566 | Mouse Anti-TXN Recombinant Antibody (VS3-CJ566) | WB | Mouse IgG1, κ |
| VS3-FY1500 | Recombinant Rabbit Anti-TXN Antibody (clone R07-4A4) | WB | Rabbit IgG |
| VS-0923-FY237 | Recombinant Mouse Anti-TXN Antibody (VS-0923-FY237) | ELISA, WB, IHC | Mouse IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-F(E) | Recombinant Anti-human TXN Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| MHH-1017-F(E) | Recombinant Human Anti-human TXN Antibody Fab Fragment | IF, FC, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1017-S(P) | Recombinant Anti-human TXN Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-1017-S(P) | Recombinant Human Anti-human TXN Antibody scFv Fragment | WB, FC, FuncS | scFv |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3728 | Rabbit Anti-TXN Recombinant Antibody (clone DS3728AB) | WB, IHC, ICC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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