Rabbit Anti-TYMS Recombinant Antibody (clone R08-2F2)
CAT#: VS3-FY1901
This R08-2F2 recombinant monoclonal antibody demonstrates high specificity in target human TYMS. The antibody is recommended for detection of TYMS protein by WB, IHC-F, IHC-P, ICC/IF, IP. The dilution ratio that is advised for optimal results is as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the thymidylate synthase antibody was determined using western blot analysis of thymidylate synthase in K562 lysates and immunocytochemistry analysis of paraffin-embedded human tonsil.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74922/168838_A_5_8_val_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74922/168838_A_7_8.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74922/168838_A_5_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74922/168838_B_6_4.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176890-TYMS
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WB
Figure 1 Anti-TYMS Antibody (VS3-FY1901) in WB.
The Thymidylate Synthase expression in K562 lysates were detected using the anti-Thymidylate Synthase by western blot assay.
ICC
Figure 2 Anti-TYMS Antibody (VS3-FY1901) in ICC.
Immunocytochemistry staining of paraffin-embedded Human tonsil using the anti-Thymidylate Synthase antibody.
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Specifications
- Immunogen
- Synthetic peptide of human thymidylate synthase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TYMS
- Species Reactivity
- Human
- Clone
- R08-2F2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thymidylate Synthetase; EC 2.1.1.45; TSase; TS; Thymidylate Synthase; HST422; TMS
- Gene ID
- 7298
- UniProt ID
- P04818
- Sequence Similarities
- Belongs to the thymidylate synthase family.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys77, Lys169, Lys104, Lys107, Lys292, and Lys308 (NX_P04818 [NX_P04818-1])
Modification sites at PhosphoSitePlus (P04818)
Glycosylation from GlyGen (P04818) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_024307010.1; XP_054175017.1; NP_001062.1; NP_001341796.1; NP_001341797.1
- Function
- Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TYMS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1404z | Mouse Anti-TYMS Recombinant Antibody (clone 4H6) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-0341MZ | Recombinant Mouse Anti-Human Thymidylate Synthase Antibody (clone US107) | IHC | Mouse antibody |
| MRO-1493-CN | Rabbit Anti-TYMS Recombinant Antibody (clone JM33-20) | WB, IHC, IP, FC | Rabbit IgG |
| ZG-0615U | Rabbit Anti-TYMS Recombinant Antibody (clone 2B2) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3733 | Rabbit Anti-TYMS Recombinant Antibody (clone DS3733AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1933 | PE-B*51:01/Human TYMS (DAHIYLNHI) MHC Tetramer | FCM | |
| MHC-YF746 | A*02:01/Human Thymidylate synthase (ALLTYMIAHI) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC961 | AbPlus™ Anti-TYMS Magnetic Beads (VS-0724-YC961) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2405 | Anti-TYMS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7663 | Anti-Mouse TYMS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7662 | Anti-Human TYMS Immunohistochemistry Kit | IHC |
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