Rabbit Anti-UBA7 Recombinant Antibody (clone R49-7X-6) (CAT#: VS3-FY2193)
Clone R49-7X-6 is a rabbit IgG recombinant monoclonal antibody that has been meticulously crafted to specifically bind to human UBA7 protein, a calculated weight of 117 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R49-7X-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), flow cytometry (dilution range 1:50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58182/1596_A3_3_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58182/1596_A3_3_selected.jpg
Figure 2 IHC staining of human fallopian tube
(Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/ihc_selected.jpg
(Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_B_7_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_B_7_5.jpg
Figure 4 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_7_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_5_6.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15444/35984_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+ RasG12V, HEL, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182179-UBA7
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182179-UBA7
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat UBA7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R49-7X-6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 117kDa, while the observed molecular weight stands at 112kDa.
- Related Disease
- Acute Promyelocytic Leukemia and Vexas Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Flow cytometry: 1/50
Target
- Alternative Names
- Ubiquitin Like Modifier Activating Enzyme 7; Ubiquitin-Activating Enzyme E1 Homolog; UBA7, Ubiquitin-Activating Enzyme E1; Ubiquitin-Activating Enzyme 7; UBE1L; UBE2; D8; UBA1, Ubiquitin-Activating Enzyme E1 Homolog B (Yeast)
- Gene ID
- 7318
- UniProt ID
- P41226
- Sequence Similarities
- Belongs to the ubiquitin-activating E1 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- ISGylated. (P41226-UBA7_HUMAN)
Modification sites at PhosphoSitePlus (P41226)
Modification sites at neXtProt (NX_P41226)
- Protein Refseq
- NP_003326.2
- Function
- Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3745 | Rabbit Anti-UBA7 Recombinant Antibody (clone DS3745AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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