AbPlus™ Anti-UBE2I Magnetic Beads (VS-0724-YC1363)
CAT#: VS-0724-YC1363
The AbPlus Anti-UBE2I Magnetic Beads (VS-0724-YC1363) is an innovative affinity resin which is bound with anti-UBE2I specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2I protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2I specific antibody
- Target
- UBE2I
- Immunogen
- Recombinant human UBE2I, aa 1-158.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 I; Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9); Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast); RING-Type E3 SUMO Transferase UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; UBC9; P18; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Conjugating Enzyme UbcE2A;
- Gene ID
- 7329
- UniProt ID
- P63279
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "UBE2I"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3668z | Mouse Anti-UBE2I Recombinant Antibody (clone 15B7) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MRO-1549-CN | Rabbit Anti-UBE2I Recombinant Antibody (clone CBACN-569) | WB, IF, IHC, IP, ChIP | Rabbit IgG |
| VS3-QX1212 | Mouse Anti-UBE2I Recombinant Antibody (clone 67AT1273.95.90) | WB | Mouse IgG1 |
| VS3-QX1213 | Mouse Anti-UBE2I Recombinant Antibody (clone 1D6-D9-E7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ1104 | Rabbit Anti-UBE2I Recombinant Antibody (VS3-CJ1104) | WB, ICC, IF, IHC, IP, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3755 | Rabbit Anti-UBE2I Recombinant Antibody (clone DS3755AB) | WB, FC, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2416 | Anti-UBE2I Immunohistochemistry Kit | IHC | |
| VS-0525-XY7700 | Anti-Human UBE2I Immunohistochemistry Kit | IHC |
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