AbPlus™ Anti-UBE2I Magnetic Beads (VS-0724-YC1363) (CAT#: VS-0724-YC1363)
The AbPlus Anti-UBE2I Magnetic Beads (VS-0724-YC1363) is an innovative affinity resin which is bound with anti-UBE2I specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2I protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg

Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg

Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg

Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg

Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2I specific antibody
- Target
- UBE2I
- Immunogen
- Recombinant human UBE2I, aa 1-158.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 I; Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9); Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast); RING-Type E3 SUMO Transferase UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; UBC9; P18; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Conjugating Enzyme UbcE2A;
- Gene ID
- 7329
- UniProt ID
- P63279
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3668z | Mouse Anti-UBE2I Recombinant Antibody (clone 15B7) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
VS3-QX1212 | Mouse Anti-UBE2I Recombinant Antibody (clone 67AT1273.95.90) | WB | Mouse IgG1 |
VS3-QX1213 | Mouse Anti-UBE2I Recombinant Antibody (clone 1D6-D9-E7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS7-0425-WR1080 | Mouse Anti-UBE2I Monoclonal Antibody (clone 1B10) | WB, IHC, ICC, FC | Mouse IgG1 |
VS7-0425-WR1081 | Mouse Anti-UBE2I Monoclonal Antibody (VS7-0425-WR1081) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
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