AbPlus™ Anti-UBE2I Magnetic Beads (VS-0724-YC1363) (CAT#: VS-0724-YC1363)
The AbPlus Anti-UBE2I Magnetic Beads (VS-0724-YC1363) is an innovative affinity resin which is bound with anti-UBE2I specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2I protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg

Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg

Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg

Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg

Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2I specific antibody
- Target
- UBE2I
- Immunogen
- Recombinant human UBE2I, aa 1-158.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 I; Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9); Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast); RING-Type E3 SUMO Transferase UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; UBC9; P18; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Conjugating Enzyme UbcE2A;
- Gene ID
- 7329
- UniProt ID
- P63279
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3668z | Mouse Anti-UBE2I Recombinant Antibody (clone 15B7) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
VS3-QX1212 | Mouse Anti-UBE2I Recombinant Antibody (clone 67AT1273.95.90) | WB | Mouse IgG1 |
VS3-QX1213 | Mouse Anti-UBE2I Recombinant Antibody (clone 1D6-D9-E7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS7-0425-WR1080 | Mouse Anti-UBE2I Monoclonal Antibody (clone 1B10) | WB, IHC, ICC, FC | Mouse IgG1 |
VS7-0425-WR1081 | Mouse Anti-UBE2I Monoclonal Antibody (VS7-0425-WR1081) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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