Mouse Anti-UBE2I Recombinant Antibody (clone SAIC-36A-9)
CAT#: FAMAB-1287CQ
This product is a recombinant mouse antibody that recognizes UBE2I. The antibody was purified by affinity chromatography. It can be used in Immuno-MRM, WB, ELISA.
Published Data
Gene Expression
ELISA
Figure 1 ELISA data from screening recombinant monoclonal antibodies using recombinant proteins or overexpressed lysates.
ELISA data were reduced using Prism software to calculate the 50% binding point (B50%). The B50% values should not be interpreted as absolute numbers that define mAb performance (i.e., not analogously to affinity constants); instead, the B50% values can be used as relative values to compare the performances of the mAb within this group of mAbs.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79920/2017_D9_3_cr5fc0faa29c79e_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3909/19847_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103275-UBE2I
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Specifications
- Immunogen
- Peptide GLFIIDGK conjugated to KLH.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human UBE2I
- Species Reactivity
- Human
- Clone
- SAIC-36A-9
- Applications
- Immuno-MRM, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 I; Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9); Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast); RING-Type E3 SUMO Transferase UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; UBC9; P18; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Conjugating Enzyme UbcE2A
- Gene ID
- 7329
- UniProt ID
- P63279
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone SAIC-36A-9"
- CAT
- Product Name
See other products for "UBE2I"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3668z | Mouse Anti-UBE2I Recombinant Antibody (clone 15B7) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MRO-1549-CN | Rabbit Anti-UBE2I Recombinant Antibody (clone CBACN-569) | WB, IF, IHC, IP, ChIP | Rabbit IgG |
| VS3-QX1212 | Mouse Anti-UBE2I Recombinant Antibody (clone 67AT1273.95.90) | WB | Mouse IgG1 |
| VS3-QX1213 | Mouse Anti-UBE2I Recombinant Antibody (clone 1D6-D9-E7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ1104 | Rabbit Anti-UBE2I Recombinant Antibody (VS3-CJ1104) | WB, ICC, IF, IHC, IP, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3755 | Rabbit Anti-UBE2I Recombinant Antibody (clone DS3755AB) | WB, FC, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1363 | AbPlus™ Anti-UBE2I Magnetic Beads (VS-0724-YC1363) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2416 | Anti-UBE2I Immunohistochemistry Kit | IHC | |
| VS-0525-XY7700 | Anti-Human UBE2I Immunohistochemistry Kit | IHC |
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