Rabbit Anti-UBE2L3 Recombinant Antibody (clone R02-4B2) (CAT#: VS3-FY2099)

• Immunogen
• Synthetic peptide of human Ube2L3/UBCH7

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat UBE2L3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R02-4B2

• Applications
• Western Blot, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 18 kDa, while the observed molecular weight stands at 18 kDa.

• Related Disease
• Papilloma and Systemic Lupus Erythematosus

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Ubiquitin Conjugating Enzyme E2 L3; Ubiquitin-Conjugating Enzyme E2-F1; E2 Ubiquitin-Conjugating Enzyme L3; Ubiquitin Carrier Protein L3; Ubiquitin-Protein Ligase L3; L-UBC; UBCH7; Ubiquitin-Conjugating Enzyme E2L 3; Ubiquitin Conjugating Enzyme E2L 3

• Gene ID
• 7332

• UniProt ID
• P68036

• Sequence Similarities
• Belongs to the ubiquitin-conjugating enzyme family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Ubiquitinated.
  The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  Autoubiquitinated in vitro (PubMed:22496338).
  (P68036-UB2L3_HUMAN)
  Ubiquitination at Lys20, Lys64, Lys67, and Lys131 (NX_P68036 [NX_P68036-1])
  Modification sites at PhosphoSitePlus (P68036)
  Glycosylation from GlyGen (P68036) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• NP_001243284.1; NP_001243285.1; NP_003338.1

• Function
• Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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