Mouse Anti-UFD1L Recombinant Antibody (clone 2A6F3) (CAT#: VS7-0425-WR1086)
This product is a mouse IgG2b antibody that binds to human UFD1L. The antibody 2A6F3 recognizes specific amino acid domain of human UFD1L (AA: 208-307). It can be used for WB, IHC, ICC, and FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30286/1505_E7_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30286/1505_E7_1_selected.jpg
Figure 2 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/ihc_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_B_7_5.jpg
(Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_5.jpg
(Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_5.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_4_6.jpg
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_4_6.jpg
Figure 7 Skeletal muscle
(Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_B_1_6.jpg
(Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_B_1_6.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_8.jpg
(Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30287/67553_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070010-UFD1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070010-UFD1/subcellular
Specifications
- Immunogen
- Recombinant human UFD1L (AA: 208-307) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human UFD1L
- Species Reactivity
- Human
- Clone
- 2A6F3
- Molecule Class
- Npl4 Cofactor
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- UFD1
- Gene ID
- 7353
- UniProt ID
- Q92890
- Long Name
- Ubiquitin fusion degradation 1 like (yeast)
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001030324.2
- Function
- Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity).; Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1223MZ | Recombinant Mouse Anti-Mouse UFD1L Antibody (clone 6f3) | ELISA, ICC, IF, IP, WB | Mouse antibody |
| VS7-0425-WR1085 | Mouse Anti-UFD1L Recombinant Antibody (clone 4F11A4) | IHC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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