Rabbit Anti-UHRF1 Recombinant Antibody (clone R06-1C4) (CAT#: VS3-FY1609)

• Immunogen
• Recombinant human UHRF1 protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human UHRF1

• Species Reactivity
• Human

• Clone
• R06-1C4

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 90 kDa, while the observed molecular weight stands at 97 kDa.

• Related Disease
• Cancer

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• Ubiquitin Like With PHD And Ring Finger Domains 1; Ubiquitin-Like PHD And RING Finger Domain-Containing Protein 1; Inverted CCAAT Box-Binding Protein Of 90 KDa; RING-Type E3 Ubiquitin Transferase UHRF1; Nuclear Zinc Finger Protein Np95; Transcription Factor ICBP90; RING Finger Protein 106; Nuclear Protein 95; HuNp95; ICBP90; RNF106

• Gene ID
• 29128

• UniProt ID
• Q96T88

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome., Ubiquitinated; which leads to proteasomal degradation. Autoubiquitinated; interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents interaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage.

• Protein Refseq
• NP_001041666.1; NP_001276979.1; NP_001276980.1

• Function
• Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF1 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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