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UIMC1
Rabbit Anti-UIMC1 Recombinant Antibody (clone R01-0D5)

Rabbit Anti-UIMC1 Recombinant Antibody (clone R01-0D5) (CAT#: VS3-FY2076)

Name: Rabbit Anti-UIMC1 Recombinant Antibody (clone R01-0D5)
SKU: VS3-FY2076
Rating: 5 (1 reviews)

The R01-0D5 recombinant monoclonal antibody demonstrates high specificity in target human UIMC1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The antibody has the positive detection of RAP80 by immunocytochemistry analysis of RAP80 (green) in Hela and western blot analysis of RAP80 in Hela, A549, HL-60, U2OS lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1 Anti-UIMC1 Antibody (VS3-FY2076) in ICC.

Figure 1 Anti-UIMC1 Antibody (VS3-FY2076) in ICC.

Immunocytochemistry staining of RAP80 (green) in Hela using the anti-RAP80 antibody. Nuclear DNA was labeled with DAPI (shown in blue).

Figure 2 Anti-UIMC1 Antibody (VS3-FY2076) in WB.

Figure 2 Anti-UIMC1 Antibody (VS3-FY2076) in WB.

The RAP80 expression in Hela, A549, HL-60, U2OS lysates were detected using the anti-RAP80 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/403_D12_2_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_B_8_5.jpg

Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_9_3.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_9_5.jpg

Figure 5 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_4_6.jpg

Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_7_8.jpg

RNA Expression

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087206-UIMC1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human RAP80

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human UIMC1

Species Reactivity
Human

Clone
R01-0D5

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence

MW
The calculated weight of the target protein is approximately 80 kDa, while the observed molecular weight stands at 80 kDa.

Related Disease
Cancer

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Ubiquitin Interaction Motif Containing 1; Retinoid X Receptor-Interacting Protein 110; Receptor-Associated Protein 80; RAP80; Ubiquitin Interaction Motif-Containing Protein 1; Receptor Associated Protein 80; BRCA1-A Complex Subunit RAP80; X2HRIP110; RXRIP110

Gene ID
51720

UniProt ID
Q96RL1

Sequence Similarities
Belongs to the RAP80 family.

Cellular Localization
Nucleus

Post Translation Modifications
Sumoylated. (Q96RL1-UIMC1_HUMAN)
Phosphorylated upon DNA damage by ATM or ATR. (Q96RL1-UIMC1_HUMAN)
Ubiquitination at Lys20, Lys31, Lys146, Lys75, Lys90, Lys232, Lys245, Lys544, Lys567, Lys587, Lys607, Lys635, Lys374, and Lys382 (NX_Q96RL1 [NX_Q96RL1-1])
Modification sites at PhosphoSitePlus (Q96RL1)

Protein Refseq
XP_005265987.1; XP_005265989.1; XP_005265990.1; XP_006714934.1; XP_011532872.1

Function
Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products IgG Antibody Products

CAT	Product Name	Application	Type
MOR-3785	Rabbit Anti-UIMC1 Recombinant Antibody (clone DS3785AB)	WB, IHC, ICC	Rabbit IgG

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