Rabbit Anti-UIMC1 Recombinant Antibody (clone R01-0D5) (CAT#: VS3-FY2076)
The R01-0D5 recombinant monoclonal antibody demonstrates high specificity in target human UIMC1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The antibody has the positive detection of RAP80 by immunocytochemistry analysis of RAP80 (green) in Hela and western blot analysis of RAP80 in Hela, A549, HL-60, U2OS lysates.
Specific Inquiry
ICC
Figure 1 Anti-UIMC1 Antibody (VS3-FY2076) in ICC.
Immunocytochemistry staining of RAP80 (green) in Hela using the anti-RAP80 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-UIMC1 Antibody (VS3-FY2076) in WB.
The RAP80 expression in Hela, A549, HL-60, U2OS lysates were detected using the anti-RAP80 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human RAP80
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UIMC1
- Species Reactivity
- Human
- Clone
- R01-0D5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 80 kDa, while the observed molecular weight stands at 80 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitin Interaction Motif Containing 1; Retinoid X Receptor-Interacting Protein 110; Receptor-Associated Protein 80; RAP80; Ubiquitin Interaction Motif-Containing Protein 1; Receptor Associated Protein 80; BRCA1-A Complex Subunit RAP80; X2HRIP110; RXRIP110
- Gene ID
- 51720
- UniProt ID
- Q96RL1
- Sequence Similarities
- Belongs to the RAP80 family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated. (Q96RL1-UIMC1_HUMAN)
Phosphorylated upon DNA damage by ATM or ATR. (Q96RL1-UIMC1_HUMAN)
Ubiquitination at Lys20, Lys31, Lys146, Lys75, Lys90, Lys232, Lys245, Lys544, Lys567, Lys587, Lys607, Lys635, Lys374, and Lys382 (NX_Q96RL1 [NX_Q96RL1-1])
Modification sites at PhosphoSitePlus (Q96RL1)
- Protein Refseq
- XP_005265987.1; XP_005265989.1; XP_005265990.1; XP_006714934.1; XP_011532872.1
- Function
- Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "UIMC1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3785 | Rabbit Anti-UIMC1 Recombinant Antibody (clone DS3785AB) | WB, IHC, ICC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK526 | Rabbit Anti-UIMC1 Recombinant Antibody (clone JM09-67) | WB, ICC, IF, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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