Rabbit Anti-UIMC1 Recombinant Antibody (clone R01-0D5)
CAT#: VS3-FY2076
The R01-0D5 recombinant monoclonal antibody demonstrates high specificity in target human UIMC1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The antibody has the positive detection of RAP80 by immunocytochemistry analysis of RAP80 (green) in Hela and western blot analysis of RAP80 in Hela, A549, HL-60, U2OS lysates.
Tested Data
Gene Expression
ICC
Figure 1 Anti-UIMC1 Antibody (VS3-FY2076) in ICC.
Immunocytochemistry staining of RAP80 (green) in Hela using the anti-RAP80 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-UIMC1 Antibody (VS3-FY2076) in WB.
The RAP80 expression in Hela, A549, HL-60, U2OS lysates were detected using the anti-RAP80 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/403_D12_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37504/77055_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087206-UIMC1
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Specifications
- Immunogen
- Synthetic peptide of human RAP80
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human UIMC1
- Species Reactivity
- Human
- Clone
- R01-0D5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 80 kDa, while the observed molecular weight stands at 80 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitin Interaction Motif Containing 1; Retinoid X Receptor-Interacting Protein 110; Receptor-Associated Protein 80; RAP80; Ubiquitin Interaction Motif-Containing Protein 1; Receptor Associated Protein 80; BRCA1-A Complex Subunit RAP80; X2HRIP110; RXRIP110
- Gene ID
- 51720
- UniProt ID
- Q96RL1
- Sequence Similarities
- Belongs to the RAP80 family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated. (Q96RL1-UIMC1_HUMAN)
Phosphorylated upon DNA damage by ATM or ATR. (Q96RL1-UIMC1_HUMAN)
Ubiquitination at Lys20, Lys31, Lys146, Lys75, Lys90, Lys232, Lys245, Lys544, Lys567, Lys587, Lys607, Lys635, Lys374, and Lys382 (NX_Q96RL1 [NX_Q96RL1-1])
Modification sites at PhosphoSitePlus (Q96RL1)
- Protein Refseq
- XP_005265987.1; XP_005265989.1; XP_005265990.1; XP_006714934.1; XP_011532872.1
- Function
- Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "UIMC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3785 | Rabbit Anti-UIMC1 Recombinant Antibody (clone DS3785AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK526 | Rabbit Anti-UIMC1 Recombinant Antibody (clone JM09-67) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7741 | Anti-UIMC1 Immunohistochemistry Kit | IHC |
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