Rabbit Anti-USP28 Recombinant Antibody (clone R07-3K1) (CAT#: VS3-XY3537)
This antibody is a recombinant rabbit antibody targeting the USP28 (Ubiquitin specific peptidase 28), which involves in G2 DNA damage checkpoint. The antibody specifically reacts with human USP28. It has been reported for use in Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/8_E3_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/8_E3_1_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_B_9_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_8_4.jpg
Figure 5 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_2_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_8_5.jpg
Figure 7 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6779/18817_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6778/18642_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000048028-USP28
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000048028-USP28
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human USP28
- Species Reactivity
- Human
- Clone
- R07-3K1
- Applications
- WB, ICC, IF, FC
- Related Disease
- Primary Autosomal Recessive Microcephaly
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- Ubiquitin specific peptidase 28
- Gene ID
- 57646
- UniProt ID
- Q96RU2
- Long Name
- Ubiquitin specific peptidase 28
- Cellular Localization
- Nucleus, nucleoplasm
- Post Translation Modifications
- Ubiquitination at Lys243 (NX_Q96RU2 [NX_Q96RU2-1])
Modification sites at PhosphoSitePlus (Q96RU2)
Glycosylation from GlyGen (Q96RU2) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001333181.1; NP_001287958.1
- Function
- Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability.
Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN.
Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C).
In contrast, it does not deubiquitinate PLK1.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3807 | Rabbit Anti-USP28 Recombinant Antibody (clone DS3807AB) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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