Human Anti-VACV B5R Recombinant Antibody (clone 303) (CAT#: FAMAB-0018JF)
Recombinant monoclonal antibody specially binds to VACV antigens B5R. This anti-VACV B5R monoclonal antibody 303 can be wildey used as an effective postexposure prophylaxis or treatment of disease caused by orthopoxviruses.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-VACV B5R Recombinant Antibody (clone 303) (FAMAB-0018JF) in SDS-PAGE
SDS-PAGE analysis of FAMAB-0018JF in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 135-245 kDa.
SEC-HPLC
Figure 2 Human Anti-VACV B5R Recombinant Antibody (clone 303) (FAMAB-0018JF) in SEC-HPLC
The purity of FAMAB-0018JF was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
ELISA
Figure 3 Human Anti-VACV B5R Recombinant Antibody (clone 303) (FAMAB-0018JF) in ELISA
ELISA analysis of FAMAB-0018JF was performed by coating with Vaccinia virus (VACV) (strain Copenhagen) Protein B5 (His Tag).
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
WB
Figure 4 Human Anti-VACV B5R Recombinant Antibody (clone 303) (FAMAB-0018JF) in WB
Western blot analysis of FAMAB-0018JF was performed by loading Vaccinia virus (VACV) (strain Copenhagen) Protein B5 (His Tag).
FAMAB-0018JF incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
DB
Figure 5 Human Anti-VACV B5R Recombinant Antibody (clone 303) (FAMAB-0018JF) in DB
Dot Blot analysis of FAMAB-0018JF was performed by coating with Vaccinia virus (VACV) (strain Copenhagen) Protein B5 (His Tag).
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Specifications
- Immunogen
- VACV particles
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Vaccinia virus B5R
- Species Reactivity
- Human
- Clone
- 303
- Applications
- WB, ELISA, IHC
- Related Disease
- Orthopoxvirus infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The VACV B5R antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Humanized Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1032 | Recombinant Anti-VACV B5R Antibody | ELISA, WB, FuncS | IgG |
| MHH-1032 | Recombinant Human Anti-VACV B5R Antibody | FC, Neut, Funcs | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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