Mouse Anti-VIL1 Recombinant Antibody (clone PT0254)
CAT#: ZG-0172C
This product is a mouse antibody that recognizes VIL1. It can be used in a wide variety of applications, such as: IHC-P, IF.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-VIL1 Antibody (clone PT0254) in IHC
Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/1253_A3_2_selected.jpg
Normal Tissue
Figure 2 High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/ihc_selected.jpg
Normal Tissue
Figure 3 High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6885/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Low Intensity: Weak Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_4_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127831-VIL1
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Specifications
- Immunogen
- Synthesized peptide derived from human Villin
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse
- Clone
- PT0254
- Applications
- IHC-P, IF
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "VIL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3661z | Mouse Anti-VIL1 Recombinant Antibody (clone 22H5) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MRO-1582-CN | Rabbit Anti-VIL1 Recombinant Antibody (clone JU34-75) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ536 | Mouse Anti-VIL1 Recombinant Antibody (VS3-CJ536) | WB, IHC | Mouse IgG2b |
| VS3-XY1587 | Mouse Anti-VIL1 Recombinant Antibody (clone 3E5G11) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1588 | Mouse Anti-VIL1 Recombinant Antibody (clone 5E3B2) | ELISA, WB, FC, IHC, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3837 | Rabbit Anti-VIL1 Recombinant Antibody (clone DS3837AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2470 | Anti-VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7850 | Anti-Mouse VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7849 | Anti-Human VIL1 Immunohistochemistry Kit | IHC |
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