Rabbit Anti-VIL1 Recombinant Antibody (clone R07-2Y5)
CAT#: VS3-XY3539
The rabbit recombinant antibody specifically recognizes VIL1 (Villin 1), which plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. The antibody specifically reacts with human, mouse and rat VIL1. It has been tested using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/1253_A3_2_selected.jpg
Normal Tissue
Figure 2 High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/ihc_selected.jpg
Normal Tissue
Figure 3 High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6885/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Low Intensity: Weak Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_4_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127831-VIL1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VIL1
- Species Reactivity
- Human
- Clone
- R07-2Y5
- Applications
- WB, IHC-P, IP
- Related Disease
- Multicentric Castleman Disease; Contagious Pustular Dermatitis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- VIL; D2S1471
- Gene ID
- 7429
- UniProt ID
- P09327
- Long Name
- Villin 1
- Sequence Similarities
- Belongs to the villin/gelsolin family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Tyrosine phosphorylation is induced by epidermal growth factor (EGF) and stimulates cell migration (By similarity).
Phosphorylated on tyrosine residues by SRC. The unphosphorylated form increases the initial rate of actin-nucleating activity, whereas the tyrosine-phosphorylated form inhibits actin-nucleating activity, enhances actin-bundling activity and enhances actin-severing activity by reducing high Ca2+ requirements. The tyrosine-phosphorylated form does not regulate actin-capping activity. Tyrosine phosphorylation is essential for cell migration: tyrosine phosphorylation sites in the N-terminus half regulate actin reorganization and cell morphology, whereas tyrosine phosphorylation sites in the C-terminus half regulate cell migration via interaction with PLCG1.
- Protein Refseq
- NP_009058.2
- Function
- Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "VIL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2216CT | Recombinant Mouse anti-Human VIL1 Monoclonal antibody (DXXC2) | IHC-P | |
| ZG-0171C | Mouse Anti-VIL1 Recombinant Antibody (clone ABT-VIL) | IHC-P, IF | Mouse IgG |
| ZG-0172C | Mouse Anti-VIL1 Recombinant Antibody (clone PT0254) | IHC-P, IF | Mouse IgG |
| VS3-WK111 | Mouse Anti-VIL1 Recombinant Antibody (clone G11-5E) | WB, ICC, IHC, FC | Mouse IgG1 |
| VS3-FY1934 | Rabbit Anti-VIL1 Recombinant Antibody (clone R07-4A2) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3837 | Rabbit Anti-VIL1 Recombinant Antibody (clone DS3837AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2470 | Anti-VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7850 | Anti-Mouse VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7849 | Anti-Human VIL1 Immunohistochemistry Kit | IHC |
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