Rabbit Anti-VIL1 Recombinant Antibody (clone R07-2Y5) (CAT#: VS3-XY3539)
The rabbit recombinant antibody specifically recognizes VIL1 (Villin 1), which plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. The antibody specifically reacts with human, mouse and rat VIL1. It has been tested using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/1253_A3_2_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/1253_A3_2_selected.jpg
Figure 2 High expression in small intestine
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/ihc_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/ihc_selected.jpg
Figure 3 High expression in duodenum
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6885/ihc_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6885/ihc_selected.jpg
Figure 4 Colon
(Endocrine cells Staining: Low Intensity: Weak Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_3.jpg
(Endocrine cells Staining: Low Intensity: Weak Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_4.jpg
Figure 6 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_4_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_4_4.jpg
Figure 7 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127831-VIL1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127831-VIL1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VIL1
- Species Reactivity
- Human
- Clone
- R07-2Y5
- Applications
- WB, IHC-P, IP
- Related Disease
- Multicentric Castleman Disease; Contagious Pustular Dermatitis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- VIL; D2S1471
- Gene ID
- 7429
- UniProt ID
- P09327
- Long Name
- Villin 1
- Sequence Similarities
- Belongs to the villin/gelsolin family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Tyrosine phosphorylation is induced by epidermal growth factor (EGF) and stimulates cell migration (By similarity).
Phosphorylated on tyrosine residues by SRC. The unphosphorylated form increases the initial rate of actin-nucleating activity, whereas the tyrosine-phosphorylated form inhibits actin-nucleating activity, enhances actin-bundling activity and enhances actin-severing activity by reducing high Ca2+ requirements. The tyrosine-phosphorylated form does not regulate actin-capping activity. Tyrosine phosphorylation is essential for cell migration: tyrosine phosphorylation sites in the N-terminus half regulate actin reorganization and cell morphology, whereas tyrosine phosphorylation sites in the C-terminus half regulate cell migration via interaction with PLCG1.
- Protein Refseq
- NP_009058.2
- Function
- Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3837 | Rabbit Anti-VIL1 Recombinant Antibody (clone DS3837AB) | WB, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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