Rabbit Anti-VIL1 Recombinant Antibody (clone R07-4A2) (CAT#: VS3-FY1934)

• Immunogen
• Synthetic peptide of human Villin

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human VIL1

• Species Reactivity
• Human

• Clone
• R07-4A2

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 93 kDa.

• Related Disease
• Multicentric Castleman Disease and Contagious Pustular Dermatitis

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Villin 1; VIL; Villin-1; D2S1471

• Gene ID
• 7429

• UniProt ID
• P09327

• Sequence Similarities
• Belongs to the villin/gelsolin family.

• Cellular Localization
• Cell projection, Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Tyrosine phosphorylation is induced by epidermal growth factor (EGF) and stimulates cell migration ()., Phosphorylated on tyrosine residues by SRC. The unphosphorylated form increases the initial rate of actin-nucleating activity, whereas the tyrosine-phosphorylated form inhibits actin-nucleating activity, enhances actin-bundling activity and enhances actin-severing activity by reducing high Ca2+ requirements. The tyrosine-phosphorylated form does not regulate actin-capping activity. Tyrosine phosphorylation is essential for cell migration: tyrosine phosphorylation sites in the N-terminus half regulate actin reorganization and cell morphology, whereas tyrosine phosphorylation sites in the C-terminus half regulate cell migration via interaction with PLCG1.

• Protein Refseq
• NP_009058.2

• Function
• Epithelial cell-specific Ca²⁺-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

Select a product category from the dropdown menu below to view related products.