Rabbit Anti-VIL1 Recombinant Antibody (clone R07-4A2)
CAT#: VS3-FY1934
The rabbit recombinant monoclonal antibody is suitable for utilization in western blot analysis of Villin in MCF-7 lysates. It has high affinity in binding to human VIL1. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R07-4A2 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/1253_A3_2_selected.jpg
Normal Tissue
Figure 2 High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6884/ihc_selected.jpg
Normal Tissue
Figure 3 High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6885/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Low Intensity: Weak Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_7_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2452/6278_A_4_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127831-VIL1
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WB
Figure 1 Anti-VIL1 Antibody (VS3-FY1934) in WB.
The Villin expression in MCF-7 lysates were detected using the anti-Villin by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Villin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human VIL1
- Species Reactivity
- Human
- Clone
- R07-4A2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 93 kDa.
- Related Disease
- Multicentric Castleman Disease and Contagious Pustular Dermatitis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Villin 1; VIL; Villin-1; D2S1471
- Gene ID
- 7429
- UniProt ID
- P09327
- Sequence Similarities
- Belongs to the villin/gelsolin family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Tyrosine phosphorylation is induced by epidermal growth factor (EGF) and stimulates cell migration ()., Phosphorylated on tyrosine residues by SRC. The unphosphorylated form increases the initial rate of actin-nucleating activity, whereas the tyrosine-phosphorylated form inhibits actin-nucleating activity, enhances actin-bundling activity and enhances actin-severing activity by reducing high Ca2+ requirements. The tyrosine-phosphorylated form does not regulate actin-capping activity. Tyrosine phosphorylation is essential for cell migration: tyrosine phosphorylation sites in the N-terminus half regulate actin reorganization and cell morphology, whereas tyrosine phosphorylation sites in the C-terminus half regulate cell migration via interaction with PLCG1.
- Protein Refseq
- NP_009058.2
- Function
- Epithelial cell-specific Ca²⁺-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "VIL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2216CT | Recombinant Mouse anti-Human VIL1 Monoclonal antibody (DXXC2) | IHC-P | |
| VS3-CJ536 | Mouse Anti-VIL1 Recombinant Antibody (VS3-CJ536) | WB, IHC | Mouse IgG2b |
| VS3-XY1587 | Mouse Anti-VIL1 Recombinant Antibody (clone 3E5G11) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1588 | Mouse Anti-VIL1 Recombinant Antibody (clone 5E3B2) | ELISA, WB, FC, IHC, ICC | Mouse IgG2b |
| VS3-FY1546 | Recombinant Mouse Anti-VIL1 Antibody (clone 3D10-9A4-4C9) | IHC-P | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3837 | Rabbit Anti-VIL1 Recombinant Antibody (clone DS3837AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2470 | Anti-VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7850 | Anti-Mouse VIL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7849 | Anti-Human VIL1 Immunohistochemistry Kit | IHC |
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