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    Mouse Anti-XRCC5 Monoclonal Antibody (clone 5C5) (CAT#: VS7-0425-WR1138)

    This product is a mouse IgG1 antibody that binds to human XRCC5. The antibody 5C5 can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/if_selected.jpg
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/55667_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endocrine cells
    Staining: High
    Intensity: Strong
    Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining: High
    Preleptotene spermatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Round or early spermatids
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Sertoli cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_6_6.jpg
    Figure 7 Breast
    Figure 7 Breast
    (Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Myoepithelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_B_1_4.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079246-XRCC5/subcellular

    Specifications

    • Immunogen
    • Recombinant human XRCC5 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human XRCC5
    • Species Reactivity
    • Human, Mouse
    • Clone
    • 5C5
    • Molecule Class
    • Dna Repair Protein
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Systemic lupus erythematosus

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • XRCC5; Lupus Ku Autoantigen Protein P86; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 (Double-Strand-Break Rejoining); Ku86; DNA Repair Protein XRCC5; KUB2; TLAA; Nuclear Factor IV; KU80; 80kDa; ATP-Dependent DNA Helicase II 80 K
    • Long Name
    • X-Ray Repair Cross Complementing 5
    • Cellular Localization
    • Chromosome; Nucleus
    • Post Translation Modifications
    • Acetylation; ADP-ribosylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • Activator; DNA-binding; Helicase; Hydrolase

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "XRCC5"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOR-3896 Rabbit Anti-XRCC5 Recombinant Antibody (clone DS3896AB) FC, ICC, IHC-P, WB Rabbit IgG

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