Mouse Anti-XRCC5 Monoclonal Antibody (clone 5C5)
CAT#: VS7-0425-WR1138
This product is a mouse IgG1 antibody that binds to human XRCC5. The antibody 5C5 can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25813/55667_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: High
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_6_6.jpg
Normal Tissue
Figure 7 Breast
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Myoepithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_B_1_4.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4468/11773_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079246-XRCC5/subcellular
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Specifications
- Immunogen
- Recombinant human XRCC5 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human XRCC5
- Species Reactivity
- Human, Mouse
- Clone
- 5C5
- Molecule Class
- Dna Repair Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Systemic lupus erythematosus
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- XRCC5; Lupus Ku Autoantigen Protein P86; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 (Double-Strand-Break Rejoining); Ku86; DNA Repair Protein XRCC5; KUB2; TLAA; Nuclear Factor IV; KU80; 80kDa; ATP-Dependent DNA Helicase II 80 K
- Gene ID
- 7520
- UniProt ID
- P13010
- Long Name
- X-Ray Repair Cross Complementing 5
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; ADP-ribosylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_066964.1
- Function
- Activator; DNA-binding; Helicase; Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "XRCC5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1003CT | Recombinant Mouse anti-Human XRCC5 Monoclonal antibody (222) | FC, ICC, IF, IP, WB | |
| MRO-0909-CN | Rabbit Anti-XRCC5 Recombinant Antibody (clone CBACN-339) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX1252 | Mouse Anti-XRCC5 Recombinant Antibody (clone 8H1-C3-G10) | WB, IF, IP | Mouse IgG1 |
| VS-0923-FY157 | Recombinant Mouse Anti-XRCC5 Antibody (VS-0923-FY157) | ELISA, WB | Mouse IgG2a |
| VS13-YC1231 | Rabbit Anti-XRCC5 Recombinant Antibody (VS13-YC1231) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3896 | Rabbit Anti-XRCC5 Recombinant Antibody (clone DS3896AB) | FC, ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2500 | Anti-XRCC5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7968 | Anti-Mouse XRCC5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7967 | Anti-Human XRCC5 Immunohistochemistry Kit | IHC |
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