Rabbit Anti-ZBTB7A Recombinant Antibody (clone R07-5E0) (CAT#: VS3-FY2104)

• Immunogen
• Synthetic peptide of human ZBTB7A

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human ZBTB7A

• Species Reactivity
• Human

• Clone
• R07-5E0

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 61 kDa.

• Related Disease
• Macrocephaly, Neurodevelopmental Delay, Lymphoid Hyperplasia, And Persistent Fetal Hemoglobin and Sleep Apnea

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Zinc Finger And BTB Domain Containing 7A; ZBTB7A

• Gene ID
• 51341

• UniProt ID
• O95365

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Sumoylated.
  Undergoes sumoylation with SUMO1 that may regulate its transcriptional activity.
  (O95365-ZBT7A_HUMAN)
  Modification sites at PhosphoSitePlus (O95365)
  Modification sites at neXtProt (NX_O95365)

• Protein Refseq
• XP_005259627.3; XP_005259628.1; XP_054177150.1; XP_054177151.1; NP_001304919.1

• Function
• Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions.
  Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms.
  That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300.
  Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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