Rabbit Anti-ZYX Recombinant Antibody (clone R01-8C8)
CAT#: VS3-FY1943
This R01-8C8 recombinant monoclonal antibody is specifically engineered to detect human ZYX. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This ZYX antibody has been teted positive by western blot analysis of ZYX in PC-3, HUVEC lysates and immunocytochemistry analysis of ZYX (green) in Hela using ZYX antibody.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4835/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9321/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75747/159163_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75747/159163_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75747/159163_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75747/159163_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159840-ZYX/subcellular
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WB
Figure 1 Anti-ZYX Antibody (VS3-FY1943) in WB.
The ZYX expression in PC-3, HUVEC lysates were detected using the anti-ZYX by western blot assay.
ICC
Figure 2 Anti-ZYX Antibody (VS3-FY1943) in ICC.
Immunocytochemistry staining of ZYX (green) in Hela using the anti-ZYX antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human Zyxin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ZYX
- Species Reactivity
- Human, Rat
- Clone
- R01-8C8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Lipomatosis, Familial Multiple and Arrhythmogenic Right Ventricular Dysplasia, Familial, 10
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Zyxin; ZYX
- Gene ID
- 7791
- UniProt ID
- Q15942
- Sequence Similarities
- Belongs to the zyxin/ajuba family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys265 (NX_Q15942 [NX_Q15942-1])
Modification sites at PhosphoSitePlus (Q15942)
Glycosylation from GlyGen (Q15942) 24 sites, 3 O-linked glycans (24 sites)
- Protein Refseq
- XP_047276773.1; XP_047276774.1; XP_054188146.1; XP_054214943.1; XP_054214944.1
- Function
- Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ZYX"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2237z | Mouse Anti-ZYX Recombinant Antibody (clone 18A3) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-1365CT | Recombinant Mouse anti-Human ZYX Monoclonal antibody (EML2268) | ICC, IF, WB | |
| MRO-1615-CN | Rabbit Anti-ZYX Recombinant Antibody (clone JU83-12) | WB, IF, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3940 | Rabbit Anti-ZYX Recombinant Antibody (clone DS3940AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2549 | Anti-ZYX Immunohistochemistry Kit | IHC | |
| VS-0525-XY8078 | Anti-Rat ZYX Immunohistochemistry Kit | IHC |
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