Rabbit Anti-ZYX Recombinant Antibody (clone R01-8C8) (CAT#: VS3-FY1943)
This R01-8C8 recombinant monoclonal antibody is specifically engineered to detect human ZYX. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This ZYX antibody has been teted positive by western blot analysis of ZYX in PC-3, HUVEC lysates and immunocytochemistry analysis of ZYX (green) in Hela using ZYX antibody.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-ZYX Antibody (VS3-FY1943) in WB.
The ZYX expression in PC-3, HUVEC lysates were detected using the anti-ZYX by western blot assay.
ICC
Figure 2 Anti-ZYX Antibody (VS3-FY1943) in ICC.
Immunocytochemistry staining of ZYX (green) in Hela using the anti-ZYX antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human Zyxin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ZYX
- Species Reactivity
- Human, Rat
- Clone
- R01-8C8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Lipomatosis, Familial Multiple and Arrhythmogenic Right Ventricular Dysplasia, Familial, 10
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Zyxin; ZYX
- Gene ID
- 7791
- UniProt ID
- Q15942
- Sequence Similarities
- Belongs to the zyxin/ajuba family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys265 (NX_Q15942 [NX_Q15942-1])
Modification sites at PhosphoSitePlus (Q15942)
Glycosylation from GlyGen (Q15942) 24 sites, 3 O-linked glycans (24 sites)
- Protein Refseq
- XP_047276773.1; XP_047276774.1; XP_054188146.1; XP_054214943.1; XP_054214944.1
- Function
- Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2237z | Mouse Anti-ZYX Recombinant Antibody (clone 18A3) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-1365CT | Recombinant Mouse anti-Human ZYX Monoclonal antibody (EML2268) | ICC/IF, WB | |
| MRO-1615-CN | Rabbit Anti-ZYX Recombinant Antibody (clone JU83-12) | WB, IF, IHC, FC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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