Rabbit Anti-ZYX Recombinant Antibody (clone R01-8C8) (CAT#: VS3-FY1943)
This R01-8C8 recombinant monoclonal antibody is specifically engineered to detect human ZYX. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This ZYX antibody has been teted positive by western blot analysis of ZYX in PC-3, HUVEC lysates and immunocytochemistry analysis of ZYX (green) in Hela using ZYX antibody.
Specific Inquiry
WB
Figure 1 Anti-ZYX Antibody (VS3-FY1943) in WB.
The ZYX expression in PC-3, HUVEC lysates were detected using the anti-ZYX by western blot assay.
ICC
Figure 2 Anti-ZYX Antibody (VS3-FY1943) in ICC.
Immunocytochemistry staining of ZYX (green) in Hela using the anti-ZYX antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human Zyxin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ZYX
- Species Reactivity
- Human, Rat
- Clone
- R01-8C8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Lipomatosis, Familial Multiple and Arrhythmogenic Right Ventricular Dysplasia, Familial, 10
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Zyxin; ZYX
- Gene ID
- 7791
- UniProt ID
- Q15942
- Sequence Similarities
- Belongs to the zyxin/ajuba family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys265 (NX_Q15942 [NX_Q15942-1])
Modification sites at PhosphoSitePlus (Q15942)
Glycosylation from GlyGen (Q15942) 24 sites, 3 O-linked glycans (24 sites)
- Protein Refseq
- XP_047276773.1; XP_047276774.1; XP_054188146.1; XP_054214943.1; XP_054214944.1
- Function
- Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ZYX"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2237z | Mouse Anti-ZYX Recombinant Antibody (clone 18A3) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-1365CT | Recombinant Mouse anti-Human ZYX Monoclonal antibody (EML2268) | ICC/IF, WB | |
| MRO-1615-CN | Rabbit Anti-ZYX Recombinant Antibody (clone JU83-12) | WB, IF, IHC, FC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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