APC-Mouse CD1d Tetramer - α-GalCer
CAT#: MHC-YF796
This product is a tetramer of CD1d tetramer loaded with α-GalCer and fluorescent-labeled with APC. It can be used to identify mouse natural killer (NK) T cells by flow cytometry and the frequency of antigen-specific NKT cells.
Specifications
- Allele
- CD1d
- Class
- Non-classical MHC
- MHC Species
- Human
- Antigen
- Alpha-galactosylceramide (alpha-GalCer)
- Antigen Species
- Mouse
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- α-GalCer is a synthetic glycolipid which is a potent activator of iNKT cells, and a model CD1d antigen.
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Excellent Identification Of NKT Cells
This CD1d tetramer loaded with alpha-GalCer is highly effective for identifying natural killer T cells in mouse splenocytes. The APC labeling is bright, and the binding specificity to the invariant NKT cell receptor allowed for clean flow cytometry data. It is a vital tool for our immunology projects.
Robust Activation And Staining Tool
We used this reagent to assess the frequency of antigen-specific iNKT cells in our experimental models. The synthetic glycolipid loading seems very stable, providing consistent staining results across multiple experiments. It significantly helped us characterize the immune cell populations in our study.
Q&As
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What specific cell type does this CD1d tetramer detect?
A: This CD1d tetramer is loaded with alpha-galactosylceramide (alpha-GalCer) and is specifically designed to identify mouse natural killer T (NKT) cells, particularly invariant NKT (iNKT) cells that recognize glycolipid antigens presented by the non-classical MHC molecule CD1d.
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Is this product suitable for flow cytometry analysis?
A: Yes, this product is fluorescently labeled with APC and is optimized for use in flow cytometry (FCM). It allows researchers to determine the frequency and phenotype of antigen-specific NKT cells in mouse single-cell suspensions.
View the frequently asked questions answered by Creative Biolabs Support.
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See other products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF787 | PE-Human CD1d Tetramer - α-GalCer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF790 | APC-Human CD1d Tetramer - α-GalCer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF793 | PE-Mouse CD1d Tetramer - α-GalCer | FCM |
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