CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070)
CAT#: VS-0126-XU102
CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070) is a precision-engineered reagent designed for the robust detection of human CD16. This recombinant monoclonal antibody ensures superior specificity for the FcγRIII epitope, minimizing experimental variability inherent in traditional hybridoma-derived clones. Optimized for flow cytometry (FC), it provides bright and stable staining, allowing for the clear identification of NK cell populations and myeloid subsets. Its high chemical purity makes it an essential tool for researchers investigating effector cell function and the therapeutic efficacy of antibody-based immunotherapies.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Not detected
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162747-FCGR3B
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU102-1 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), AF Dye (488nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (488nm) | Synthetic green fluorogenic dye (Ex/Em ~490/520 nm). A photostable alternative to FITC with brighter signal and narrower emission spectrum, reducing spillover into PE channel. | Excellent photostability. Maintains signal integrity during prolonged acquisition. Tolerates fixation well and exhibits minimal pH sensitivity. | ||||||
| VS-0126-XU102-2 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), AF Dye (700nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (700nm) | Synthetic far-red fluorogenic dye (Ex/Em ~685/700 nm). Excels in deep-red channels with superior brightness and minimal spillover. Excellent for panels requiring high-dimensional analysis. | Exceptional photostability and fixation tolerance. Resists quenching under continuous laser exposure. Store at 4°C in the dark. | ||||||
| VS-0126-XU102-3 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), AF Dye (647nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (647nm) | Synthetic far-red fluorogenic dye (Ex/Em ~650/665 nm). Spectrally similar to APC but with enhanced brightness and photostability. Ideal for replacing APC in demanding protocols. | Superior stability. Highly resistant to photobleaching and pH fluctuations. Compatible with harsh fixation and permeabilization protocols. | ||||||
| VS-0126-XU102-4 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU102-5 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), APC-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC-Cy7 | Tandem dye (APC excited, Cy7 emits at ~785 nm). Provides a bright, far-red to infrared signal. Shares the APC-Cy7 detection channel with other Cy7 tandems. Also prone to bond degradation under stress. | Low stability. Similar sensitivity as PE-Cy7 to fixation and light. Requires strict adherence to dark, cold storage and prompt analysis. | ||||||
| VS-0126-XU102-6 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. | ||||||
| VS-0126-XU102-7 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), Pacific Blue-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Pacific Blue | Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design. | High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability. | ||||||
| VS-0126-XU102-8 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), Percp-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Percp | Protein-based fluorochrome (Ex/Em: 488/678 nm). Compatible with blue laser excitation, providing signal in red channel. Lower brightness compared to PE or APC but useful for spectral separation. | Moderate stability. PerCP is inherently photolabile and prone to degradation upon light exposure. Store strictly protected from light. | ||||||
| VS-0126-XU102-9 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU102-10 | CytoStream™ Mouse Anti-CD16 Recombinant Antibody (MMC-M1070), Percp-Cy5.5-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Percp-Cy5.5 | Tandem dye (PerCP excited, Cy5.5 emits at ~694 nm). Compatible with blue laser (488 nm) excitation. PerCP component is less bright but provides distinct spectral signature. Tandem bond susceptible to degradation. | Low to moderate stability. PerCP itself is photolabile; tandem format adds sensitivity to fixation. Analyze promptly after staining. | ||||||
| VS-0126-XU102-11 | CytoStream™ Purified Mouse Anti-CD16 Recombinant Antibody (MMC-M1070) | 100 ug | None | The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection. | Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer. |
More Infomation
Specifications
- Host Species
- Mouse
- Type
- Monoclonal Antibody
- Specificity
- CD16
- Species Reactivity
- Human
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 10 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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