Accelerated™ Fluorescent-Antibody Labeling Kit (VS-0824-FY127)
CAT#: VS-0824-FY127
Accelerated™ fluorescent antibody labeling kit can be effectively accomplished in just 20 minutes, requiring only 30 seconds of active hands-on time when utilizing the Biotinylation kit/Biotin binding kit. This process ensures 100% recovery of antibodies.
| Sub Cat | Fluorescent Dye Name | Product Name | |
| VS-0824-FY9 | Biotin | Accelerated™ Biotin-Antibody Labeling Kit | |
| VS-0824-FY10 | FITC | Accelerated™ FITC-Antibody Labeling Kit | |
| VS-0824-FY11 | Cy5 | Accelerated™ Cy5-Antibody Labeling Kit | |
| VS-0824-FY16 | Cy3 | Accelerated™ Cy3-Antibody Labeling Kit | |
| VS-0824-FY20 | Texas Red | Accelerated™ Texas Red-Antibody Labeling Kit | |
| VS-0824-FY21 | Rhodamine | Accelerated™ Rhodamine-Antibody Labeling Kit | |
| VS-0824-FY34 | Cy5.5 | Accelerated™ Cy5.5-Antibody Labeling Kit | |
| VS-0824-FY37 | AMCA | Accelerated™ AMCA-Antibody Labeling Kit | |
| VS-0824-FY12 | Atto 488 | Accelerated™ Atto 488-Antibody Labeling Kit | |
| VS-0824-FY32 | Atto 565 | Accelerated™ Atto 565-Antibody Labeling Kit | |
| VS-0824-FY33 | Atto 633 | Accelerated™ Atto 633-Antibody Labeling Kit | |
| VS-0824-FY36 | Atto 390 | Accelerated™ Atto 390-Antibody Labeling Kit | |
| VS-0824-FY14 | DyLight 488 | Accelerated™ DyLight 488-Antibody Labeling Kit | |
| VS-0824-FY17 | DyLight 594 | Accelerated™ DyLight 594-Antibody Labeling Kit | |
| VS-0824-FY18 | DyLight 650 | Accelerated™ DyLight 650-Antibody Labeling Kit | |
| VS-0824-FY19 | DyLight 550 | Accelerated™ DyLight 550-Antibody Labeling Kit | |
| VS-0824-FY23 | Dylight 350 | Accelerated™ Dylight 350-Antibody Labeling Kit | |
| VS-0824-FY24 | DyLight 405 | Accelerated™ DyLight 405-Antibody Labeling Kit | |
| VS-0824-FY27 | DyLight 633 | Accelerated™ DyLight 633-Antibody Labeling Kit | |
| VS-0824-FY28 | DyLight 680 | Accelerated™ DyLight 680-Antibody Labeling Kit | |
| VS-0824-FY31 | DyLight 755 | Accelerated™ DyLight 755-Antibody Labeling Kit | |
| VS-0824-FY35 | DyLight 800 | Accelerated™ DyLight 800-Antibody Labeling Kit |
More Infomation
Specifications
- Fluorescent Dye Name
- Accelerated™ Fluorescent Dyes
- Application
- Western Blot, ELISA, Immunohistochemistry, etc
- Composition
- Fluorescent conjugation (Biotinylation Kit / Biotin or FITC or Cy5 or Atto 488 or DyLight 488 or Cy3 or DyLight 594 or DyLight 650 or DyLight 550 or Texas red or Rhodamine or Dylight 350 or DyLight 405 or DyLight 633 or DyLight 680 or FluoProbes 647H or DyLight 755 or Atto 565 or Atto 633 or Cy5.5 or DyLight 800 or Atto 390 or AMCA)
Modifier reagent
Quencher reagent
- Procedure Steps
- Assay Procedure
Equilibrate all materials and prepared reagents to room temperature before use.
1. Add 1 µL of modifier per 10 µL of antibody to be labeled and mix gently.
2. Remove the cap from the vial of biotin conjugation mixture and drop the antibody sample (with modifier) directly onto the lyophilized material. Resuspend by gently aspirating and redispensing the liquid once or twice using a pipette.
3. Cap the vial and incubate at room temperature (20-25°C) in the dark for 15 minutes. Longer incubation times (e.g., overnight) have no negative effect on the conjugation.
4. After 15 minutes (or longer) of incubation, add 1 µL of quencher per 10 µL of antibody and mix gently. The conjugate is ready for use after 5 minutes. The conjugate does not require purification.
Please refer to the COA of the shipped product.
- Product Precautions
- Recommended pre-conjugation buffer components and conditions:
The antibody should be a purified antibody.
Antibody concentration can be 0.5-2 mg/mL.
pH can be 6.5-8.5.
Amine free buffer (e.g. MES, MOPS, HEPES, PBS)
Non-buffering salts (e.g. sodium chloride)
BSA <0.1%
Chelating agents (e.g. EDTA) <0.1%
Glycerol <50%
Sugars
Gelatin <0.1%
Tris <50mM
Borate buffer
Buffer components & conditions should not include the following:
Antibody in ascites fluid, serum, hybridoma or tissue culture media
Glycine / Thimerosal / Thimerosal / Merthiolate / Proclin
Nucleophilic components (Primary amines e.g. amino acids or ethylenediamine and thiols e.g. mercaptoethanol or DTT)
Note: Individually, these components should not affect the reaction. In combination, however, the reaction may be affected.
This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Store kit at -20°C immediately upon receipt.
- Conjugate Storage
- 1. Antibody conjugates can be stored at 4°C for up to 18 months. For longer storage, conjugates can be stored at -20°C in a cryoprotectant such as 50% glycerol.
2. Optimal storage conditions for any particular conjugate must be determined experimentally and will depend on antibody stability and optimal storage conditions.
3. Antibody conjugates should always be stored in the dark.
- Assay Platform
- Fluorescence microscope
- Sample Type
- Only antibodies that have been purifed are appropriate for use, meaning that antibodies found in ascites fluid, serum, or hybridoma culture media are not suitable due to the presence of other proteins, peptides, or amino acids.
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- Fluorescent dyes are a type of molecule that emit light when excited by ultraviolet or visible light. Accelerated fluorescent dyes are a particular group of fluorescent dyes that have a rapid response time, allowing for quick and reliable detection of target molecules or compounds in a variety of applications.
These dyes are often used in imaging and detection technologies such as fluorescence microscopy, flow cytometry, and DNA sequencing, as their rapid fluorescence response enables real-time analysis of biological samples with high sensitivity and resolution.
Accelerated fluorescent dyes are also used in various other fields such as materials science, environmental monitoring, and clinical diagnostics, where their quick response time and high signal-to-noise ratio make them a valuable tool for detecting and quantifying specific targets in complex samples.
Overall, accelerated fluorescent dyes play a critical role in advancing research and development in a wide range of fields, providing researchers with a powerful and versatile tool for studying and analyzing biological processes and materials.
- Alter Name
- Accelerated™ Biotin-Antibody Labeling Kit; Accelerated™ FITC-Antibody Labeling Kit; Accelerated™ Cy5-Antibody Labeling Kit; Accelerated™ Atto 488-Antibody Labeling Kit; Accelerated™ DyLight 488-Antibody Labeling Kit; Accelerated™ Cy3-Antibody Labeling Kit; Accelerated™ DyLight 594-Antibody Labeling Kit; Accelerated™ DyLight 650-Antibody Labeling Kit; Accelerated™ DyLight 550-Antibody Labeling Kit; Accelerated™ Texas Red-Antibody Labeling Kit; Accelerated™ Rhodamine-Antibody Labeling Kit; Accelerated™ Dylight 350-Antibody Labeling Kit; Accelerated™ DyLight 405-Antibody Labeling Kit; Accelerated™ DyLight 633-Antibody Labeling Kit; Accelerated™ DyLight 680-Antibody Labeling Kit; Accelerated™ FluoProbes 647H-Antibody Labeling Kit; Accelerated™ DyLight 755-Antibody Labeling Kit; Accelerated™ Atto 565-Antibody Labeling Kit; Accelerated™ Atto 633-Antibody Labeling Kit; Accelerated™ Cy5.5-Antibody Labeling Kit; Accelerated™ DyLight 800-Antibody Labeling Kit; Accelerated™ Atto 390-Antibody Labeling Kit; Accelerated™ AMCA-Antibody Labeling Kit
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