Quick™ Fluorescent-Antibody Labeling Kit (VS-0824-FY128) (CAT#: VS-0824-FY128)

• Fluorescent Dye Name
• Quick Fluorescent Dyes

• Composition
• Fluorescent conjugation mix
  Modifier reagent
  Quencher reagent

• Procedure Steps
• Assay Procedure
  Equilibrate all materials and prepared reagents to room temperature before use.
  1. Add 1 µL of modifier per 10 µL of antibody to be labeled and mix gently.
  2. Remove the cap from the vial of fluorescent conjugation mixture and drop the antibody sample (with modifier) ​​directly onto the lyophilized material. Resuspend by gently aspirating and redispensing the liquid once or twice using a pipette.
  3. Cap the vial and incubate at room temperature (20-25°C) in the dark for 3 hours. Longer incubation times (e.g., overnight) have no negative effect on the conjugation.
  4. After 3 hours (or longer) of incubation, add 1 µL of quencher per 10 µL of antibody and mix gently. The conjugate is ready for use after 30 minutes. The conjugate does not require purification.
  Please refer to the COA of the shipped product.

• Product Precautions
• Recommended pre-conjugation buffer components and conditions:
  The antibody should be a purified antibody.
  Antibody concentration can be 0.5-1 mg/mL.
  pH can be 6.5-8.5.
  Amine free buffer (e.g. MES, MOPS, HEPES, PBS)
  Non-buffering salts (e.g. sodium chloride)
  BSA <0.1%
  Chelating agents (e.g. EDTA) <0.1%
  Glycerol <50%
  Sugars
  Gelatin <0.1%
  Tris <50mM
  Borate buffer
  Buffer components & conditions should not include the following:
  Antibody in ascites fluid, serum, hybridoma or tissue culture media
  Glycine / Thimerosal / Thimerosal / Merthiolate / Proclin
  Nucleophilic components (Primary amines e.g. amino acids or ethylenediamine and thiols e.g. mercaptoethanol or DTT)
  Note: Individually, these components should not affect the reaction. In combination, however, the reaction may be affected.
  This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.

• Kit Storage
• Store kit at -20°C immediately upon receipt.

• Conjugate Storage
• 1. Antibody conjugates can be stored at 4°C for up to 18 months. For longer storage, conjugates can be stored at -20°C in a cryoprotectant such as 50% glycerol.
  2. Optimal storage conditions for any particular conjugate must be determined experimentally and will depend on antibody stability and optimal storage conditions.
  3. Antibody conjugates should always be stored in the dark.

• Assay Platform
• Fluorescence microscope

• Sample Type
• Only antibodies that have been purifed are appropriate for use, meaning that antibodies found in ascites fluid, serum, or hybridoma culture media are not suitable due to the presence of other proteins, peptides, or amino acids.

• Reactive Species
• The reactive species of the kit depends on the research application of the antibody.

• Fluorescent Dye Introduction
• Fluorescent dyes are a type of molecule that emit light when excited by ultraviolet or visible light. Accelerated fluorescent dyes are a particular group of fluorescent dyes that have a rapid response time, allowing for quick and reliable detection of target molecules or compounds in a variety of applications.
  These dyes are often used in imaging and detection technologies such as fluorescence microscopy, flow cytometry, and DNA sequencing, as their rapid fluorescence response enables real-time analysis of biological samples with high sensitivity and resolution.
  Accelerated fluorescent dyes are also used in various other fields such as materials science, environmental monitoring, and clinical diagnostics, where their quick response time and high signal-to-noise ratio make them a valuable tool for detecting and quantifying specific targets in complex samples.
  Overall, accelerated fluorescent dyes play a critical role in advancing research and development in a wide range of fields, providing researchers with a powerful and versatile tool for studying and analyzing biological processes and materials.

• Alter Name
• Quick™ APC-Antibody Labeling Kit; Quick™ FITC-Antibody Labeling Kit; Quick™ PE/R-PE-Antibody Labeling Kit; Quick™ HRP-Antibody Labeling Kit; Quick™ APC/Cy7-Antibody Labeling Kit; Quick™ PerCP/Cy5.5-Antibody Labeling Kit; Quick™ PE/Cy7-Antibody Labeling Kit; Quick™ PE/Cy5-Antibody Labeling Kit; Quick™ APC/Cy5.5-Antibody Labeling Kit; Quick™ ALP-Antibody Labeling Kit; Quick™ Streptavidin-Antibody Labeling Kit; Quick™ APC/Cy5.5-Antibody Labeling Kit; Quick™ PerCP-Antibody Labeling Kit; Quick™ PE/Texas Red-Antibody Labeling Kit; Quick™ ALP (Animal Free)-Antibody Labeling Kit; Quick™ B-Phycoerythrin-Antibody Labeling Kit; Quick™ Glucose Oxidase-Antibody Labeling Kit; Quick™ PE/Atto 594-Antibody Labeling Kit; Quick™ Cy7-Antibody Labeling Kit; Quick™ R-PE-Antibody Labeling Kit

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