Human Anti-F2RL1 (clone Ab4999) scFv-Fc Chimera
CAT#: VS-0325-FY119
The anti-F2RL1 scFv-Fc antibody targets F2RL1 (PAR2), a protease-activated receptor involved in inflammation, pain signaling, and cancer biology. It is useful for studying the role of F2RL1 in cell signaling, immune responses, and disease pathogenesis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/14091/1740_E4_17_cr57fce65847914_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_B_8_5.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_5_1.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12989/33496_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (PC-3, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164251-F2RL1
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Specifications
- Immunogen
- The initial antibody was generated through phage display methods to identify Fab fragments that specifically bind to the residues surrounding the protease cleavage site at the N-terminal of PAR-2.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- This antibody exhibits binding specificity for the N-terminal peptide of PAR-2.
- Species Reactivity
- Human
- Clone
- Ab4999
- Applications
- Blocking, ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- F2R Like Trypsin Receptor 1; Coagulation Factor II (Thrombin) Receptor-Like 1; G-Protein Coupled Receptor 11; Thrombin Receptor-Like 1; GPR11; PAR2
- Gene ID
- 2150
- UniProt ID
- P55085
- Long Name
- F2R Like Trypsin Receptor 1
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- N-glycosylated and sialylated.
Multiple phosphorylated on serine and threonine residues in the cytoplasmic region upon receptor activation; required for receptor desensitization and recruitment of beta-arrestin.
Monoubiquitinated by CBL at the plasma membrane and in early endosomes; not required for receptor endocytosis but for translocation to late endosomes or lysosomes.
Deubiquitination involves STAMBP and USP8; required for lysosomal trafficking and receptor degradation.
Glycosylation at Asn30, Thr45, Ser46, Thr49, Thr54, and Asn222
Ubiquitination at Lys368
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 7 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- NP_005233.4
- Function
- Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection.
Generally is promoting inflammation.
Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling.
Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X.
Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone Ab4999"
- CAT
- Product Name
See other products for "F2RL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1591z | Mouse Anti-F2RL1 Recombinant Antibody (clone 38D1) | WB, FC, IF, IHC, IP | Mouse IgG2a, κ |
| HPAB-0363-CN | Human Anti-F2RL1 Recombinant Antibody (HPAB-0363-CN) | WB, FC, Block | Human IgG |
| HPAB-0364-CN | Human Anti-F2RL1 Recombinant Antibody (HPAB-0364-CN) | WB, FC, Block | Human IgG |
| HPAB-1526-FY | Human Anti-F2RL1 Recombinant Antibody (clone Ab3777) | Block | Human IgG |
| HPAB-1527-FY | Human Anti-F2RL1 Recombinant Antibody (clone Ab4213) | Block | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0934CL | Human Anti-F2RL1 Recombinant Antibody (TAB-0934CL) | ELISA, RIA, FC | Human IgG, κ |
| TAB-0935CL | Human Anti-F2RL1 Recombinant Antibody (TAB-0935CL) | ELISA, RIA, FC | Human IgG, κ |
| TAB-0934CL-S(P) | Human Anti-F2RL1 Recombinant Antibody; scFv Fragment (TAB-0934CL-S(P)) | ELISA, RIA, FC | Human scFv |
| TAB-0934CL-F(E) | Human Anti-F2RL1 Recombinant Antibody; Fab Fragment (TAB-0934CL-F(E)) | ELISA, RIA, FC | Human Fab |
| TAB-0935CL-F(E) | Human Anti-F2RL1 Recombinant Antibody; Fab Fragment (TAB-0935CL-F(E)) | ELISA, RIA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0610CLV | Mouse Anti-F2RL1 Recombinant Antibody (TAB-0610CLV) | Inhib, IF, WB | Mouse IgG |
| TAB-0610CLV-F(E) | Mouse Anti-F2RL1 Recombinant Antibody; Fab Fragment (TAB-0610CLV-F(E)) | ELISA | Mouse Fab |
| TAB-0610CLV-S(P) | Mouse Anti-F2RL1 Recombinant Antibody; scFv Fragment (TAB-0610CLV-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0363-CN-S(P) | Human Anti-F2RL1 Recombinant Antibody; scFv Fragment (HPAB-0363-CN-S(P)) | WB, FC, Block | Human scFv |
| HPAB-0364-CN-S(P) | Human Anti-F2RL1 Recombinant Antibody; scFv Fragment (HPAB-0364-CN-S(P)) | WB, FC, Block | Human scFv |
| HPAB-1526-FY-F(E) | Human Anti-F2RL1 Recombinant Antibody (clone Ab3777); scFv Fragment | Block | Human scFv |
| HPAB-1527-FY-F(E) | Human Anti-F2RL1 Recombinant Antibody (clone Ab4213); scFv Fragment | Block | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0363-CN-F(E) | Human Anti-F2RL1 Recombinant Antibody; Fab Fragment (HPAB-0363-CN-F(E)) | WB, FC, Block | Human Fab |
| HPAB-0364-CN-F(E) | Human Anti-F2RL1 Recombinant Antibody; Fab Fragment (HPAB-0364-CN-F(E)) | WB, FC, Block | Human Fab |
| HPAB-1526-FY-S(P) | Human Anti-F2RL1 Recombinant Antibody (clone Ab3777); Fab Fragment | Block | Human Fab |
| HPAB-1527-FY-S(P) | Human Anti-F2RL1 Recombinant Antibody (clone Ab4213); Fab Fragment | Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC371 | CytoStream™ Rabbit Anti-F2RL1 Recombinant Antibody (VS13-YC371) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC284 | Recombinant Anti-F2RL1 Vesicular Antibody, EV Displayed (VS-0425-YC284) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2410 | Anti-F2RL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2411 | Anti-Mouse F2RL1 Immunohistochemistry Kit | IHC |
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