Human Anti-GLB1 scFv-Fc Chimera (VS-0325-FY95)
CAT#: VS-0325-FY95
The anti-β-galactosidase scFv-Fc antibody is a recombinant antibody designed to target β-galactosidase, an enzyme commonly used as a reporter gene in molecular biology and biochemistry. The single-chain variable fragment (scFv) fused to the Fc region of an immunoglobulin allows for enhanced stability, solubility, and effective effector functions, making it a valuable tool for various applications in research and therapeutic settings.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- AMEF strain GLB1
- Species Reactivity
- AMEF strain
- Applications
- Immunoassay
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Galactosidase Beta 1; Elastin Receptor 1, 67kDa; Acid Beta-Galactosidase; EC 3.2.1.23; Lactase; ELNR1; Elastin Receptor 1 (67kD)
- Gene ID
- 2720
- UniProt ID
- P16278
- Long Name
- Galactosidase Beta 1
- Sequence Similarities
- Belongs to the glycosyl hydrolase 35 family.
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Glycosylation at Asn26, Asn247, Asn464, Asn498, Asn542, Asn545, and Asn555
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect BGAL_HUMAN (1036)
Glycosylation from GlyGen 8 sites, 81 N-linked glycans (4 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000395.3; NP_001073279.2; NP_001129074.2; NP_001303969.2; NP_001380509.1
- Function
- AMEF strain GLB1 refers to a particular strain of Acanthamoeba, which is a free-living amoeba. Specific characteristics of the GLB1 strain, often including its pathogenic potential and genetic makeup, are important in understanding Acanthamoeba infections.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-75 | Mouse Anti-GLB1 Recombinant Antibody (clone 13R4); Fab Fragment | WB, FC | Mouse Fab |
| PFBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody Fab Fragment (13R4) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-77 | Hamster Anti-GLB1 Recombinant Antibody (clone 13R4) | WB, FC | Hamster IgG |
| PABL-361 | Recombinant Mouse Anti-β-galactosidase Antibody (13R4) | WB, ELISA, FuncS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody scFv Fragment (13R4) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1450 | Hi-Affi™ Rabbit Anti-GLB1 Recombinant Antibody (clone DS1450AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY221 | Mouse Anti-NHP GLB1 Recombinant Antibody (clone OTI1C9) | WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| VS13-YC450 | CytoStream™ Rabbit Anti-GLB1 Recombinant Antibody (VS13-YC450) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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