Human Anti-GLB1 scFv-Fc Chimera (VS-0325-FY95) (CAT#: VS-0325-FY95)
The anti-β-galactosidase scFv-Fc antibody is a recombinant antibody designed to target β-galactosidase, an enzyme commonly used as a reporter gene in molecular biology and biochemistry. The single-chain variable fragment (scFv) fused to the Fc region of an immunoglobulin allows for enhanced stability, solubility, and effective effector functions, making it a valuable tool for various applications in research and therapeutic settings.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Figure 5 Thyroid gland
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- AMEF strain GLB1
- Species Reactivity
- AMEF strain
- Applications
- Immunoassay
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Galactosidase Beta 1; Elastin Receptor 1, 67kDa; Acid Beta-Galactosidase; EC 3.2.1.23; Lactase; ELNR1; Elastin Receptor 1 (67kD)
- Gene ID
- 2720
- UniProt ID
- P16278
- Long Name
- Galactosidase Beta 1
- Sequence Similarities
- Belongs to the glycosyl hydrolase 35 family.
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Glycosylation at Asn26, Asn247, Asn464, Asn498, Asn542, Asn545, and Asn555
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect BGAL_HUMAN (1036)
Glycosylation from GlyGen 8 sites, 81 N-linked glycans (4 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000395.3; NP_001073279.2; NP_001129074.2; NP_001303969.2; NP_001380509.1
- Function
- AMEF strain GLB1 refers to a particular strain of Acanthamoeba, which is a free-living amoeba. Specific characteristics of the GLB1 strain, often including its pathogenic potential and genetic makeup, are important in understanding Acanthamoeba infections.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| DrMAB-75 | Mouse Anti-GLB1 Recombinant Antibody (clone 13R4); Fab Fragment | WB, FC | Mouse Fab |
| PFBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody Fab Fragment (13R4) | WB, ELISA, FuncS | Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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