Mouse Anti-NHP GLB1 Recombinant Antibody (clone OTI1C9) (CAT#: VS-1024-XY221)
This mouse monoclonal antibody provides precise detection of GLB1, with reactivity across dog, human, and non-human primate samples. Utilizing a full-length recombinant human GLB1 protein as the immunogen, it ensures high specificity and reliable results. Ideal for Western blot (WB) and immunohistochemistry (IHC), this antibody is a valuable tool for investigating GLB1 expression and its role in diverse research and diagnostic applications.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Figure 5 Thyroid gland
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
Specifications
- Immunogen
- Full length human recombinant protein of GLB1
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- GLB1
- Species Reactivity
- Dog, Human, Non-human primate
- Clone
- OTI1C9
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500, IHC: 1:200
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| DrMAB-75 | Mouse Anti-GLB1 Recombinant Antibody (clone 13R4); Fab Fragment | WB, FC | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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